Bucana C D, Trial J, Papp A C, Wu K K
Department of Cell Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Scanning Microsc. 1988 Sep;2(3):1559-66.
Interleukin 2 induced alterations in the morphology of bovine aortic endothelial cells in vitro. The changes observed in confluent cultures of bovine aortic endothelial cells included retraction and elongation of cells leading to enlarged gaps between cells quantified by image analysis. Purified IL-2 (1 U/ml medium) increased the gaps between endothelial cells 3-4-fold compared with control cultures. The effect was transient, since the cells reverted to their original morphology 6-12 hours after the removal of IL-2. Correlative scanning electron microscopy (SEM) studies using fresh bovine aorta showed a dose-dependent alteration of the endothelial surface by IL-2 characterized by rounding and elongation of endothelial cells and prominent perinuclear areas. Gaps between the endothelial cells were observed when aorta samples were incubated with 2 U of IL-2/ml of medium. This was confirmed by SEM, transmission electron microscopy and Evans blue dye staining. These results suggest that IL-2 caused morphological alterations in endothelial cells that enhanced the permeability of the vascular endothelium.
白细胞介素2在体外诱导牛主动脉内皮细胞形态发生改变。在牛主动脉内皮细胞汇合培养物中观察到的变化包括细胞收缩和伸长，导致细胞间间隙增大，通过图像分析进行量化。与对照培养物相比，纯化的白细胞介素2（1单位/毫升培养基）使内皮细胞间的间隙增加了3至4倍。这种作用是短暂的，因为在去除白细胞介素2后6至12小时，细胞恢复到原来的形态。使用新鲜牛主动脉进行的相关扫描电子显微镜（SEM）研究显示，白细胞介素2对内皮表面有剂量依赖性改变，其特征为内皮细胞变圆和伸长以及明显的核周区域。当主动脉样本与2单位白细胞介素2/毫升培养基一起孵育时，观察到内皮细胞之间存在间隙。这通过扫描电子显微镜、透射电子显微镜和伊文思蓝染料染色得到证实。这些结果表明，白细胞介素2引起内皮细胞形态改变，增强了血管内皮的通透性。
