Gilboa N, Neumann P H, Del Vecchio P J, Gudewicz P W
New York State Department of Health, Wadsworth Center for Laboratories and Research, Albany 12201.
Biochem Int. 1988 Sep;17(3):585-92.
Exposure of bovine pulmonary artery endothelial cells (BEC) to polymorphonuclear leukocytes (PMN) (1 x 10(6)/ml) stimulated by opsonized zymosan (OZ-PMN) decreased plasminogen activator (PA) activity in conditioned medium by as much as 98% as compared to controls. Unstimulated PMN (U-PMN) (1 x 10(6)/ml), after an initial enhancement, decreased PA activity 95%. Low concentrations of U-PMN and OZ-PMN enhanced and high concentrations depressed PA activity. The decline in PA activity was not associated with an increased endothelial cell injury. The suppression of PA activity by OZ-PMN was associated with a 7-fold increase in activity of PA inhibitor 1 (PAI-1). PMN did not affect PA after its secretion, suggesting that modulation of endothelial cells by PMN changes the net activities of PA and PAI-1 in the conditioned medium.
与对照相比，将牛肺动脉内皮细胞（BEC）暴露于经调理酵母聚糖刺激的多形核白细胞（PMN）（1×10⁶/ml）（OZ - PMN），可使条件培养基中的纤溶酶原激活物（PA）活性降低多达98%。未刺激的PMN（U - PMN）（1×10⁶/ml）在最初增强后，使PA活性降低95%。低浓度的U - PMN和OZ - PMN增强PA活性，高浓度则抑制PA活性。PA活性的下降与内皮细胞损伤增加无关。OZ - PMN对PA活性的抑制与PA抑制剂1（PAI - 1）活性增加7倍有关。PMN分泌PA后不影响其活性，这表明PMN对内皮细胞的调节改变了条件培养基中PA和PAI - 1的净活性。
