State Key laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, State-province Joint Engineering Laboratory of Targeted Drugs from Natural Products, School of Life Sciences, Xiamen University, Xiamen, Fujian, China; Cancer Research Center of Xiamen University, Xiamen, Fujian, P. R. China.
Faculty of Medical Technology, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Acta Trop. 2020 Nov;211:105623. doi: 10.1016/j.actatropica.2020.105623. Epub 2020 Jul 6.
Oncomelania hupensis is the obligate intermediate host of Schistosoma japonicum, and it also serves as the first intermediate host for Exorchis sp., which uses Parasilurus asoyus as its definitive host rather than humans. In previous studies, Tang et al. found that all S. japonicum larvae can be blocked and killed in O. hupensis pre-infected with Exorchis sp. eggs. However, the molecular and cellular mechanisms involved in this process remain unclear. Therefore, in the present study, a combined transcriptomic and proteomic analysis was performed to identify the differential proteins involved in the immune response to the parasite S. japonicum in the O. hupensis snail host pre-infected with Exorchis sp. trematodes. The results showed that a total of 46,162 unigenes were obtained with 23,535 (50.98%) unigenes annotated in relevant databases, and 3811 proteins from O. hupensis were identified. In addition, iTRAQ-based quantitative proteomic analysis demonstrated that among three groups (OhSj-1_vs_OhN-1, OhE-1_vs_OhN-1 and OhES-1_vs_OhN-1), there were 146 common differential proteins including 44 up-regulated proteins and 90 down-regulated proteins, and 195 differential proteins exclusive to only one experimental group, including 91 up-regulated proteins and 104 down-regulated proteins, which were defined as the Common group and the Only group, respectively. KEGG analysis showed that 15 and 11 differential proteins were annotated in "Infectious diseases" in the Common group and the Only group, respectively, indicating that these proteins may be involved in the snail host immune response to parasite infection. These data will be helpful for better understanding the host-parasite interaction, and could pave the way towards exploring the mechanisms involved in the biological control on S. japonicum in O. hupensis. They also provide valuable information about developing new anti-schistosomiasis strategies.
钉螺是日本血吸虫的唯一中间宿主，也是异形科吸虫的第一中间宿主，而异形科吸虫的终末宿主是平胸龟，而不是人类。在以前的研究中，Tang 等人发现，所有的日本血吸虫幼虫都可以在感染了异形科吸虫卵的钉螺中被阻断和杀死。然而，这一过程中涉及的分子和细胞机制尚不清楚。因此，本研究采用联合转录组和蛋白质组学分析方法，鉴定感染异形科吸虫的钉螺宿主中抗寄生虫日本血吸虫免疫反应相关的差异蛋白。结果表明，共获得 46162 条 unigenes，其中 23535 条（50.98%）unigenes在相关数据库中得到注释，鉴定出 3811 种钉螺蛋白。此外，iTRAQ 定量蛋白质组学分析表明，在三组（OhSj-1_vs_OhN-1、OhE-1_vs_OhN-1 和 OhES-1_vs_OhN-1）中，共有 146 个共同差异蛋白，包括 44 个上调蛋白和 90 个下调蛋白，以及仅在一个实验组中存在的 195 个差异蛋白，包括 91 个上调蛋白和 104 个下调蛋白，分别定义为共同组和仅一组。KEGG 分析表明，共同组和仅一组中分别有 15 个和 11 个差异蛋白被注释为"传染病"，表明这些蛋白可能参与了钉螺宿主对寄生虫感染的免疫反应。这些数据将有助于更好地理解宿主-寄生虫的相互作用，并为探索钉螺中日本血吸虫的生物控制机制铺平道路。它们还为开发新的抗血吸虫病策略提供了有价值的信息。
