Huber K L, Cousins R J
Food Science and Human Nutrition Department, University of Florida, Gainesville 32611.
J Nutr. 1988 Dec;118(12):1570-6. doi: 10.1093/jn/118.12.1570.
The influence of maternal dietary zinc intake and recombinant human interleukin-1 alpha (rhIL-1 alpha) administration on metallothionein gene expression and the distribution of 65Zn were investigated. Pregnant rats were fed diets containing 1, 5, 30 or 180 mg Zn/kg diet in an equalized regime from d 13-20 of gestation. Metallothionein gene expression was examined by Northern blot and dot blot hybridization using combined 60-mer oligonucleotides specific for rat metallothionein-1 and -2 genes. Expression was progressively depressed in the fetal livers and livers and kidneys of dams fed diets marginal (5 mg/kg) and deficient (1 mg/kg) in zinc content. Administration of rhIL-1 alpha increased expression in maternal liver, placenta and in fetal liver of dams fed adequate or deficient diets. Kinetics of intravenously administered 65Zn showed that in response to rhIL-1 alpha, there was a higher uptake by the maternal liver and bone marrow with less 65Zn uptake by bone, intestine and plasma activity compared to controls. No change was observed in 65Zn taken up by the placenta or transferred to the fetus. Alteration of metallothionein gene expression could represent, in part, the mechanism whereby altered effects of zinc metabolism and function are mediated during fetal development.
研究了母体膳食锌摄入量和重组人白细胞介素-1α（rhIL-1α）给药对金属硫蛋白基因表达及65Zn分布的影响。在妊娠第13至20天，给怀孕大鼠喂食锌含量分别为1、5、30或180mg/kg的均衡日粮。使用针对大鼠金属硫蛋白-1和-2基因的60聚体寡核苷酸组合，通过Northern印迹和斑点印迹杂交检测金属硫蛋白基因表达。在喂食锌含量处于边缘水平（5mg/kg）和缺乏水平（1mg/kg）日粮的母鼠的胎儿肝脏、肝脏和肾脏中，基因表达逐渐降低。给喂食充足或缺乏日粮的母鼠注射rhIL-1α可增加母体肝脏、胎盘和胎儿肝脏中的表达。静脉注射65Zn的动力学表明，与对照组相比，对rhIL-1α的反应是，母体肝脏和骨髓摄取量更高，而骨骼、肠道和血浆活性摄取的65Zn更少。胎盘摄取或转移至胎儿的65Zn未观察到变化。金属硫蛋白基因表达的改变可能部分代表了在胎儿发育过程中介导锌代谢和功能改变效应的机制。
