Zartner Luisa, Garni Martina, Craciun Ioana, Einfalt Tomaž, Palivan Cornelia G
Department of Chemistry, University of Basel, Mattenstrasse 24a, BPR 1096, P.O. Box 3350, CH-4002 Basel, Switzerland.
Department of Pharmaceutical Sciences, Division of Pharmaceutical Technology, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
Biomacromolecules. 2021 Jan 11;22(1):106-115. doi: 10.1021/acs.biomac.0c00624. Epub 2020 Jul 28.
Cellular model systems are essential platforms used across multiple research fields for exploring the fundaments of biology and biochemistry. Here, we present giant plasma membrane vesicles (GPMVs) as a platform of cell-like compartments that will facilitate the study of particles within a biorelevant environment and promote their further development. We studied how cellularly taken up nanoparticles (NPs) can be transferred into formed GPMVs and which are the molecular factors that play a role in successful transfer (size, concentration, and surface charge along with 3 different cell lines: HepG2, HeLa, and Caco-2). We observed that polystyrene (PS) carboxylated NPs with a size of 40 and 100 nm were successfully and efficiently transferred to GPMVs derived from all cell lines. We then investigated the distribution of NPs inside formed GPMVs and established the average number of NPs/GPMVs and the percentage of all GPMVs with NPs in their cavity. We pave the way for GPMV usage as superior cell-like mimics in medically relevant applications.
细胞模型系统是多个研究领域用于探索生物学和生物化学基础的重要平台。在此，我们展示了巨型质膜囊泡（GPMV）作为一种细胞样隔室平台，它将有助于在生物相关环境中研究颗粒并促进其进一步发展。我们研究了细胞摄取的纳米颗粒（NP）如何转移到形成的GPMV中，以及在成功转移中起作用的分子因素（尺寸、浓度和表面电荷，以及3种不同的细胞系：HepG2、HeLa和Caco-2）。我们观察到，尺寸为40和100 nm的聚苯乙烯（PS）羧化NP成功且高效地转移到了源自所有细胞系的GPMV中。然后，我们研究了NP在形成的GPMV内的分布，并确定了每个GPMV内NP的平均数量以及腔内含有NP的所有GPMV的百分比。我们为GPMV在医学相关应用中作为优质细胞样模拟物的使用铺平了道路。
