Fish Genetics and Biotechnology Division, ICAR-Central Institute of Fisheries Education, Mumbai, India.
Animal Biotechnology Department, Anand Agricultural University, Anand, India.
Genomics. 2020 Nov;112(6):4041-4052. doi: 10.1016/j.ygeno.2020.07.012. Epub 2020 Jul 8.
Transcriptome analysis of Clarias magur brain and gonads at preparatory, mature, 6 and 16 h post-GnRH injection (hpi) stages yielded 9.5 GB data with 39,738 contigs. Sequences of 45 reproductive genes were identified for the first time in C. magur along with unique and differentially expressed genes. The expression of 20 genes was validated by qRT-PCR. Upregulation of Cyp11A1, Cyp17A1 and FTZF1 genes in the 16hpi testis accompanied by the 17β-HSD3 expression indicates testosterone (T) synthesis in response to LH surge, while reduced expression of CYP11B1 suggests a high T: 11-KT ratio. It is evident by the gene expression analysis that the inhibitory neurotransmitter GABA, altered T: 11-KT, increased testicular bile acids, and oxytocin-like neuropeptide in the male brain, appear to be involved in arresting the pulsatile motion of testicular smooth muscles. The work generates important leads for an effective induced breeding strategy for silurid catfish.
在预备期、成熟期、注射 GnRH 后 6 和 16 小时（hpi）的阶段，对胡子鲶脑和性腺进行转录组分析，得到了 9.5GB 的数据，其中包含 39738 个连续序列。首次在胡子鲶中鉴定到了 45 个生殖基因，以及独特和差异表达的基因。通过 qRT-PCR 验证了 20 个基因的表达。在 16 hpi 的睾丸中，Cyp11A1、Cyp17A1 和 FTZF1 基因的上调以及 17β-HSD3 的表达表明，LH 激增导致了睾酮（T）的合成，而 CYP11B1 的表达减少表明 T:11-KT 比值较高。基因表达分析表明，在雄性大脑中，抑制性神经递质 GABA、改变的 T:11-KT、增加的睾丸胆汁酸和催产素样神经肽似乎参与了阻止睾丸平滑肌的脉冲运动。这项工作为有效诱导鲶鱼的繁殖策略提供了重要线索。
