College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, Heilongjiang 161006, PR China.
College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, Heilongjiang 161006, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Nov 5;241:118658. doi: 10.1016/j.saa.2020.118658. Epub 2020 Jul 3.
Research on pyrophosphate ions detection remains important because it plays crucial roles in various fields. A simple and new colorimetric sensor for pyrophosphate (PPi) based on mono-pyrenylurea ligand (L) has been designed and synthesized by a simple reaction of 1-pyrenemethylamine hydrochloride with p-nitrophenylisocyanate. In DMSO-15% HO solution and DMSO-15% HEPES (10 mM, pH = 7.2) buffer solution, L displayed a selective colorimetric response for pyrophosphate (PPi) against other anions by changing color from colorless to yellow. This recognition process was confirmed by UV-vis spectroscopy. Also, the colorimetric properties of L are attributed to the anion-induced deprotonation of the urea subunit as demonstrated by H NMR titration method. Moreover, convenient test strips coated with L could be utilized to detect PPi in aqueous solution by naked-eye.
焦磷酸盐离子检测的研究仍然很重要，因为它在各个领域都起着至关重要的作用。本文通过 1- （ 对 - 硝基苯甲酰基 ） 氨基 - 吡咯烷与对 - 硝基异氰酸苯酯的简单反应，设计并合成了一种基于单 - 吡咯基脲配体（ L ）的简单新的焦磷酸盐（ PPi ）比色传感器。在 DMSO-15% HO 溶液和 DMSO-15% HEPES （ 10 mM ， pH = 7.2 ）缓冲溶液中， L 对焦磷酸盐（ PPi ）表现出选择性的比色响应，颜色由无色变为黄色。这一识别过程通过紫外 - 可见光谱得到了证实。此外，通过核磁共振滴定法证明， L 的比色性质归因于脲亚基的阴离子诱导去质子化。此外，涂有 L 的方便试条可用于通过肉眼检测水溶液中的 PPi 。
