BANCR 表达水平的升高对伴有 BRAFV600E 突变的甲状腺乳头状癌的进展有不同的影响。

Elevated BANCR expression levels have different effects on papillary thyroid carcinoma progression depending on the presence of the BRAFV600E mutation.

机构信息

Institute for the Application of Nuclear Energy - INEP, University of Belgrade, Belgrade, Serbia.

Center for Endocrine Surgery, Institute of Endocrinology, Diabetes and Diseases of Metabolism, Clinical Center of Serbia, Belgrade, Serbia.

出版信息

Eur J Surg Oncol. 2020 Oct;46(10 Pt A):1835-1842. doi: 10.1016/j.ejso.2020.05.027. Epub 2020 Jun 18.

DOI:10.1016/j.ejso.2020.05.027

PMID:32651027

Abstract

INTRODUCTION

The role of BRAF-activated non-protein coding RNA (BANCR) in papillary thyroid carcinoma (PTC) is controversial, its clinical significance is unclear and no study has correlated the presence of the BRAFV600E mutation in PTC with BANCR expression.

METHODS

BANCR levels in PTC and matched nonmalignant thyroid epithelial tissues from 85 patients were determined using quantitative RT-PCR. BRAFV600E was detected by mutant allele-specific PCR amplification. The results were correlated with clinicopathological characteristics of the patients.

RESULTS

The presence of BRAFV600E associates with lower relative BANCR expression (RBE) in PTC (p = 0.008). RBE is down-regulated in BRAFV600E positive PTC, while it is unchanged or up-regulated in BRAFV600E negative PTC compared to the levels in paired nonmalignant tissue (p = 0.001). At the cut-off of 31.3%, sensitivity of fold change of BANCR for the presence of BRAFV600E is 68.0% and specificity is 67.2%. In BRAFV600E positive PTC up-regulated BANCR predicts lymph node metastasis (p = 0.001), while in BRAFV600E negative PTCs high RBE predicts thyroid capsule invasion (p = 0.028).

CONCLUSIONS

Depending on the presence of BRAFV600E, elevated BANCR levels demonstrated different effects on lymphatic spreading and local PTC invasion. Therefore, BANCR could be a useful prognostic biomarker in risk stratification of PTC patients.

摘要

简介

BRAF 激活的非编码 RNA（BANCR）在甲状腺乳头状癌（PTC）中的作用存在争议，其临床意义尚不清楚，也没有研究将 PTC 中的 BRAFV600E 突变与 BANCR 表达相关联。

方法

采用定量 RT-PCR 法检测 85 例 PTC 及配对非癌甲状腺上皮组织中 BANCR 的水平。采用突变等位基因特异性 PCR 扩增法检测 BRAFV600E。将结果与患者的临床病理特征相关联。

结果

BRAFV600E 的存在与 PTC 中相对 BANCR 表达（RBE）较低相关（p=0.008）。与配对非癌组织相比，BRAFV600E 阳性 PTC 中 BANCR 的 RBE 下调，而 BRAFV600E 阴性 PTC 中 BANCR 的 RBE 不变或上调（p=0.001）。在 31.3%的截断值下，BANCR 折叠变化对 BRAFV600E 存在的敏感性为 68.0%，特异性为 67.2%。在 BRAFV600E 阳性的 PTC 中，上调的 BANCR 预示着淋巴结转移（p=0.001），而在 BRAFV600E 阴性的 PTC 中，高 RBE 预示着甲状腺包膜侵犯（p=0.028）。