Medical Science Research Centre, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, P.R. China.
Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, P.R. China.
Oncol Rep. 2020 Aug;44(2):519-532. doi: 10.3892/or.2020.7626. Epub 2020 May 27.
Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. Circular RNAs (circRNAs) are a novel class of RNAs, with higher stability and tissue specificity, which may be of value as novel clinical markers. High‑throughput RNA sequencing was used to profile the expression of circRNAs in 5 pairs of cancer and normal tissues, and reverse transcription‑quantitative PCR (RT‑qPCR) analysis was employed to verify the results of the RNA sequencing in 45 cases of PTC. The dysregulated circRNA expression and clinicopathological characteristics were assessed and the potential roles of circRNAs in the cellular miRNA and mRNA network were predicted using bioinformatics analysis. The results demonstrated that, compared with normal tissues, a total of 53 circRNAs were dysregulated in tumour tissues, and 8 circRNAs were validated at the mRNA level (P<0.001 and P<0.01). Among those, the expression of chr5:161330882‑161336769‑ (P=0.015), chr9:22046750‑22097364+ (P=0.041) and chr8:18765448‑18804898‑ (P=0.036) were obviously associated with the BRAFV600E mutation, chr12:129699809‑129700698‑ was associated with capsular invasion (P=0.025) and chr5:38523418‑38530666‑ was associated with pT stage (P=0.037) and lymph node metastasis (P=0.002). Therefore, some dysregulated circRNAs were found to be associated with BRAFV600E mutation, capsular invasion, advanced pT stage and lymph node metastasis of PTC, indicating that circRNAs may be involved in tumourigenesis and cancer progression, and they may be putative biomarkers for the diagnosis and evaluation of progression of PTC.
甲状腺癌（PTC）是最常见的甲状腺癌类型。环状 RNA（circRNA）是一种新型 RNA，具有更高的稳定性和组织特异性，可能作为新型临床标志物具有价值。使用高通量 RNA 测序来描绘 5 对癌组织和正常组织中 circRNA 的表达，并使用逆转录-定量 PCR（RT-qPCR）分析在 45 例 PTC 中验证 RNA 测序的结果。通过生物信息学分析评估了失调的 circRNA 表达与临床病理特征，并预测了 circRNA 在细胞 miRNA 和 mRNA 网络中的潜在作用。结果表明，与正常组织相比，肿瘤组织中共有 53 个 circRNA 表达失调，在 mRNA 水平验证了 8 个 circRNA（P<0.001 和 P<0.01）。其中，chr5：161330882-161336769-（P=0.015）、chr9：22046750-22097364+（P=0.041）和 chr8：18765448-18804898-（P=0.036）的表达与 BRAFV600E 突变明显相关，chr12：129699809-129700698-与包膜侵犯相关（P=0.025），chr5：38523418-38530666-与 pT 分期（P=0.037）和淋巴结转移（P=0.002）相关。因此，一些失调的 circRNA 与 BRAFV600E 突变、包膜侵犯、晚期 pT 分期和淋巴结转移相关，表明 circRNA 可能参与肿瘤发生和癌症进展，并且可能是 PTC 诊断和进展评估的潜在生物标志物。
