Taktak Y S, Grant A M
Department of Chemical Pathology, Old Medical School, Leeds University, United Kingdom.
Horm Metab Res. 1988 Nov;20(11):682-6. doi: 10.1055/s-2007-1010916.
The rat hepatoma cell line MH1C1 has been characterized to show a stimulated secretion of C-reactive protein in response to both leukocyte supernatant and a purified human interleukin-1 preparation. The time-dependency and dose-response relationship of CRP secretion were comparable to and somewhat more sensitive than the effects of leukocyte supernatant and purified human interleukin-1 on the proliferative rate of murine thymocytes; the proliferative rate of the hepatoma cell line MH1C1 was unchanged under these conditions. Agents which affect the thymocyte bioassay response to interleukin-1 namely interleukin-2, lipopolysaccharide, concanavalin A and phytohemagglutinin showed no effect on the C-reactive protein release of the MH1C1 cell line. These data strongly support the suitability of this cell line for the in vitro study of the hepatic acute phase stimulus-secretion response.
大鼠肝癌细胞系MH1C1的特点是，对白细胞上清液和纯化的人白细胞介素-1制剂均有反应，可刺激C反应蛋白的分泌。C反应蛋白分泌的时间依赖性和剂量反应关系与白细胞上清液和纯化的人白细胞介素-1对小鼠胸腺细胞增殖率的影响相当，且在某种程度上更敏感；在这些条件下，肝癌细胞系MH1C1的增殖率没有变化。影响胸腺细胞对白介素-1生物测定反应的试剂，即白介素-2、脂多糖、刀豆球蛋白A和植物血凝素，对MH1C1细胞系的C反应蛋白释放没有影响。这些数据有力地支持了该细胞系适用于肝急性期刺激-分泌反应的体外研究。
