评估对羟基苯乙酮对体内 DNA 彗星试验中 DNA 损伤水平的影响。
Evaluation of the Effect of p-Tyrosol on the Level of DNA Damage in the DNA Comet Assay In Vivo.
机构信息
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Tomsk, Russia.
Cancer Research Institute, Tomsk National Research Medical Center, Tomsk, Russia.
出版信息
Bull Exp Biol Med. 2020 Jun;169(2):233-236. doi: 10.1007/s10517-020-04857-y. Epub 2020 Jul 10.
The effect of p-tyrosol on the spontaneous level of DNA damage in the cells of the bone marrow, liver, kidney, and rectum of mice (series I) and on the genotoxic effects of cytostatic drugs with different mechanisms of action in rat testicular cells (series II) was studied by DNA comet assay on C57BL/6 mice. p-Tyrosol was administered in a dose of 40 mg/kg once (series I) or for 5 days before and 5 days after cytostatic exposure (busulfan, paclitaxel, methotrexate; series II). It was found that p-tyrosol reduced spontaneous level of DNA damage in all studied organs. p-Tyrosol exhibited an antigenotoxic effect with respect to the DNA-damaging action of methotrexate and produced no genoprotective effect in case of busulfan and paclitaxel.
研究了 p-对羟苯乙醇对 C57BL/6 小鼠骨髓、肝脏、肾脏和直肠细胞中 DNA 损伤自发水平（系列 I）以及对具有不同作用机制的细胞抑制剂药物对大鼠睾丸细胞中遗传毒性影响（系列 II）的作用。通过 DNA 彗星试验进行了研究。p-对羟苯乙醇以 40mg/kg 的剂量单次给药（系列 I）或在细胞抑制剂暴露前 5 天和后 5 天给药（白消安、紫杉醇、甲氨蝶呤；系列 II）。结果发现，p-对羟苯乙醇降低了所有研究器官中的 DNA 损伤自发水平。p-对羟苯乙醇对甲氨蝶呤的 DNA 损伤作用具有抗原毒性作用，而对白消安和紫杉醇则没有产生遗传保护作用。
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