Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, College of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province, 210023, China.
Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, College of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province, 210023, China; Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University, Jinan, Shandong Province, 250014, China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu Province, 222005, China.
Fish Shellfish Immunol. 2020 Oct;105:152-163. doi: 10.1016/j.fsi.2020.06.045. Epub 2020 Jul 8.
In insects, Taiman (Tai) participates in the juvenile hormone, 20-hydroxyecdysone, insulin, and Hippo signaling pathways. However, the role of Tai in crustacean innate immunity is less known. In this study, four Tai isoforms (MnTai-A, MnTai-B, MnTai-C, and MnTai-D) produced by alternative splicing were identified from Macrobrachium nipponense. The obtained genome sequences indicated that MnTai DNA has more than 20 exons and 19 introns. The second to last (-exon2) and the third to last (-exon3) exons can be alternatively spliced. The loss of -exon2 or -exon3 produces MnTai-B or MnTai-C, respectively. Both exons are absent in MnTai-D. The full-length cDNA of MnTai-A (including all exons) was 6894 bp with an open reading frame of 4998 bp that encoded a protein of 1665 amino acids. MnTaiA contains the conservative structure of the Tai family and clustered with nuclear receptor coactivator from shrimp. All these four isoforms were widely distributed in a variety of tissues with the highest expression level in the hepatopancreas except MnTaiC. The transcriptional levels of total Tai genes (designated as MnTaiT) in the hepatopancreas and gills were regulated by bacterial or viral challenge. Knockdown of MnTaiT increased the expression of anti-microbial peptides (AMPs) during Vibrio parahaemolyticus infection. Further study indicated that the negative regulation of AMP gene expression by prawn Tai was mediated through its positive regulation of cactus. Our research provides valuable information that prawn Tai isoforms are involved in innate immunity.
在昆虫中，Tai（Tai）参与了保幼激素、20-羟基蜕皮酮、胰岛素和 Hippo 信号通路。然而，Tai 在甲壳动物先天免疫中的作用知之甚少。在本研究中，从日本沼虾中鉴定出了四个通过选择性剪接产生的 Tai 异构体（MnTai-A、MnTai-B、MnTai-C 和 MnTai-D）。获得的基因组序列表明，MnTai DNA 具有超过 20 个外显子和 19 个内含子。倒数第二个（-exon2）和倒数第三个（-exon3）外显子可以进行选择性剪接。失去-exon2 或-exon3 分别产生 MnTai-B 或 MnTai-C。MnTai-D 中两个外显子均不存在。MnTai-A 的全长 cDNA（包括所有外显子）为 6894 bp，开放阅读框为 4998 bp，编码 1665 个氨基酸的蛋白质。MnTaiA 含有 Tai 家族的保守结构，并与虾的核受体共激活因子聚类。这四个异构体均广泛分布于多种组织中，除 MnTaiC 外，肝胰腺中的表达水平最高。肝胰腺和鳃中总 Tai 基因（命名为 MnTaiT）的转录水平受细菌或病毒挑战的调节。MnTaiT 的敲低增加了副溶血弧菌感染期间抗菌肽（AMPs）的表达。进一步的研究表明，对虾 Tai 对 AMP 基因表达的负调控是通过其对 cactus 的正调控来介导的。我们的研究提供了有价值的信息，表明对虾 Tai 异构体参与先天免疫。
