环状 RNA circEIF3M 通过促进细胞周期蛋白 D1 的表达促进乳腺癌的进展。
The circular RNA circEIF3M promotes breast cancer progression by promoting cyclin D1 expression.
机构信息
Department of General Surgery, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China.
Department of Pathology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, China.
出版信息
Aging (Albany NY). 2020 Jul 11;12(14):14775-14790. doi: 10.18632/aging.103539.
We investigated the function of circular RNA circEIF3M (hsa_circ_0003119) in triple-negative breast cancer. The expression profiles of circRNAs in 3 specimens of triple-negative breast cancer tissues with adjacent nontumor tissues were analyzed by RNA-sequencing. We verified the oncogenic role of circEIF3M in triple-negative breast cancer through a series of biological function experiments. It was found that circEIF3M was markedly upregulated in triple-negative breast cancer as compared to adjacent nontumor tissue, and that circEIF3M promoted triple-negative breast cancer cell proliferation, migration, and invasion. Mechanistic analysis indicated that circEIF3M may act as a competing endogenous RNA for miR-33a that relieves the inhibitory effect of miR-33a on its target cyclin D1. These findings showed that circEIF3M promotes triple-negative breast cancer progression via the circEIF3M/ miR-33a/ cyclin D1 axis.
我们研究了环状 RNA circEIF3M（hsa_circ_0003119）在三阴性乳腺癌中的功能。通过 RNA 测序分析了 3 例三阴性乳腺癌组织及其相邻非肿瘤组织中 circRNAs 的表达谱。我们通过一系列生物学功能实验验证了 circEIF3M 在三阴性乳腺癌中的致癌作用。结果发现，与相邻非肿瘤组织相比，circEIF3M 在三阴性乳腺癌中明显上调，并且 circEIF3M 促进三阴性乳腺癌细胞的增殖、迁移和侵袭。机制分析表明，circEIF3M 可能作为 miR-33a 的竞争性内源性 RNA，解除 miR-33a 对其靶标 cyclin D1 的抑制作用。这些发现表明，circEIF3M 通过 circEIF3M/miR-33a/cyclin D1 轴促进三阴性乳腺癌的进展。
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