抗反转录病毒疗法对 HIV 患者 IL-6、IL-1β、TNF-α、IFN-γ 水平的影响及其与 HIV-RNA 和 CD4+ T 细胞的关系。

The Effect of Antiretroviral Therapy on IL-6, IL-1β, TNF-α, IFN-γ Levels and their Relationship with HIV-RNA and CD4+ T Cells in HIV Patients.

机构信息

Bezmialem Vakif University, Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Istanbul, Turkey

Bezmialem Vakif University, Faculty of Medicine, Department of Medical Biochemistry, Istanbul, Turkey

出版信息

Curr HIV Res. 2020;18(5):354-361. doi: 10.2174/1570162X18666200712174642.

DOI:10.2174/1570162X18666200712174642

PMID:32652911

Abstract

BACKGROUND

Serum cytokine levels over the course of HIV infection usually increase with immunosuppression and decrease after antiretroviral treatment (ART).

OBJECTIVES

The aim of the study is to compare cytokine levels between HIV-infected patients (HIP) and controls and investigate the relationship between CD4+T cell count, HIV-RNA levels, and cytokine levels.

METHODS

The study subjects comprised ART-naive HIP (n=30) with no comorbidities and age-and sex-matched healthy controls. We measured levels of IL-6, IL-1β, TNF-α, and IFN-γ in serum samples of HIP at the beginning and at month 6 of ART and in controls.

RESULTS

The mean age of the study subjects was 38.7 ±10.3 years, with men making up 86.7% of the study subjects (n=26). IL-6, IL-1β, and TNF-α levels were significantly higher in both ART-naive (p<0.001, p=0.002, p=0.001) and ART-experienced HIP (p<0.001) than controls. The IFN-γ level was lower in both ART-naive and ART-experienced HIP compared to controls (p=0.082 and p=0.002). There was a positive correlation between the CD4+T cell count and serum concentration of IFN- γ(r=0.320, p<0.05). While the serum IFN-γ concentration showed a negative correlation with the HIVRNA level(r=-0.412, p<0.001), the serum IL-1β, IL-6, and TNF-α concentrations showed a positive correlation with the HIV-RNA level (r=0.349, p<0.001; r:0.54, p<0.001; r:0.438, p<0.00).

CONCLUSION

Although serum concentrations of IL-6, IL-1β and TNF-α showed a significant decrease after ART, they were still significantly higher than the controls. IFN-γ responded differently to ART compared to the other cytokines, indicating that it may play a distinct and important role in the pathogenesis of HIV infection.

摘要

背景

在 HIV 感染过程中，血清细胞因子水平通常随着免疫抑制的增加而升高，并在抗逆转录病毒治疗 (ART) 后降低。

目的

本研究旨在比较 HIV 感染患者 (HIP) 与对照组之间的细胞因子水平，并探讨 CD4+T 细胞计数、HIV-RNA 水平与细胞因子水平之间的关系。

方法

研究对象包括未经 ART 治疗的初治 HIP（n=30）和年龄、性别匹配的健康对照组。我们在 ART 开始时和第 6 个月测量了 HIP 血清样本中 IL-6、IL-1β、TNF-α 和 IFN-γ 的水平。

结果

研究对象的平均年龄为 38.7±10.3 岁，其中男性占研究对象的 86.7%（n=26）。初治和有 ART 治疗史的 HIP 中，IL-6、IL-1β 和 TNF-α 水平均显著升高（均 p<0.001，p=0.002，p=0.001）。与对照组相比，初治和有 ART 治疗史的 HIP 中 IFN-γ 水平均较低（p=0.082 和 p=0.002）。CD4+T 细胞计数与 IFN-γ 血清浓度呈正相关（r=0.320，p<0.05）。而血清 IFN-γ 浓度与 HIVRNA 水平呈负相关（r=-0.412，p<0.001），血清 IL-1β、IL-6 和 TNF-α 浓度与 HIV-RNA 水平呈正相关（r=0.349，p<0.001；r：0.54，p<0.001；r：0.438，p<0.001）。