利用计算机平均技术重建谷氨酰胺合成酶。
Reconstruction of glutamine synthetase using computer averaging.
作者信息
Frank J, Goldfarb W, Eisenberg D, Baker T S
出版信息
Ultramicroscopy. 1978;3(3):283-90. doi: 10.1016/s0304-3991(78)80038-2.
Abstract
The axial projection of the glutamine synthetase molecule has been reconstructed from electron micrographs of a stained preparation by using a new method of correlation search and averaging. The average over 50 individual molecules appears as a radial pattern with sixfold symmetry. The handedness evident in the average is attributed to nonuniformity of the negative stain.
摘要
通过一种新的相关搜索和平均方法，从染色制剂的电子显微照片中重建了谷氨酰胺合成酶分子的轴向投影。50个单个分子的平均值呈现出具有六重对称性的径向模式。平均值中明显的旋向性归因于负染的不均匀性。
相似文献
1
Reconstruction of glutamine synthetase using computer averaging.利用计算机平均技术重建谷氨酰胺合成酶。
Ultramicroscopy. 1978;3(3):283-90. doi: 10.1016/s0304-3991(78)80038-2.
2
Addendum to "reconstruction of glutamine synthetase using computer averaging".《“利用计算机平均法重建谷氨酰胺合成酶”的补遗》
Ultramicroscopy. 1979;04(2):247. doi: 10.1016/s0304-3991(79)90319-x.
3
Thermostabilization of Bacillus caldolyticus glutamine synthetase by intrinsic and extrinsic factors.内在和外在因素对嗜热解淀粉芽孢杆菌谷氨酰胺合成酶的热稳定性影响
Curr Top Cell Regul. 1985;26:263-80. doi: 10.1016/b978-0-12-152826-3.50027-9.
4
Averages of glutamine synthetase molecules as obtained with various skin and electron dose conditions.在不同皮肤和电子剂量条件下获得的谷氨酰胺合成酶分子的平均值。
J Supramol Struct. 1980;14(4):405-22. doi: 10.1002/jss.400140402.
5
Localization of the site of adenylylation of glutamine synthetase by electron microscopy of an enzyme-antibody complex.通过酶-抗体复合物的电子显微镜观察对谷氨酰胺合成酶腺苷酸化位点进行定位
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5778-82. doi: 10.1073/pnas.75.12.5778.
6
Regulation of the activity of the Bacillus licheniformis A5 glutamine synthetase.地衣芽孢杆菌A5谷氨酰胺合成酶活性的调控
J Bacteriol. 1981 Oct;148(1):174-82. doi: 10.1128/jb.148.1.174-182.1981.
7
Time-resolved low-dose microscopy of glutamine synthetase molecules.谷氨酰胺合成酶分子的时间分辨低剂量显微镜检查
Ultramicroscopy. 1984;13(3):241-52. doi: 10.1016/0304-3991(84)90203-1.
8
Glutamine synthetase forms three- and seven-stranded helical cables.谷氨酰胺合成酶形成三股和七股螺旋束。
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3402-6. doi: 10.1073/pnas.72.9.3402.
9
Electron microscopy of glutamine synthetase from pea leaf chloroplasts.豌豆叶叶绿体谷氨酰胺合成酶的电子显微镜观察
Biochim Biophys Acta. 1980 Nov 20;626(1):1-4. doi: 10.1016/0005-2795(80)90190-7.
10
[Electron microscopy of glutamine synthetase from pea leaf chloroplasts].[豌豆叶叶绿体谷氨酰胺合成酶的电子显微镜观察]
Biokhimiia. 1981 Jan;46(1):29-32.
引用本文的文献
1
Cryo-EM elucidates the uroplakin complex structure within liquid-crystalline lipids in the porcine urothelial membrane.冷冻电镜解析了猪尿路上皮膜中液晶脂质内尿路上皮蛋白复合结构。
Commun Biol. 2023 Oct 7;6(1):1018. doi: 10.1038/s42003-023-05393-x.
2
Novel Artificial Intelligence-Based Approaches for Ab Initio Structure Determination and Atomic Model Building for Cryo-Electron Microscopy.基于新型人工智能的从头算结构测定和冷冻电子显微镜原子模型构建方法
Micromachines (Basel). 2023 Aug 27;14(9):1674. doi: 10.3390/mi14091674.
3
Unveiling Liquid-Crystalline Lipids in the Urothelial Membrane through Cryo-EM.通过冷冻电镜揭示尿路上皮细胞膜中的液晶脂质
Res Sq. 2023 Jul 12:rs.3.rs-3080731. doi: 10.21203/rs.3.rs-3080731/v1.
4
Unveiling Liquid-Crystalline Lipids in the Urothelial Membrane through Cryo-EM.通过冷冻电镜揭示尿路上皮膜中的液晶脂质
bioRxiv. 2023 Aug 6:2023.05.29.542358. doi: 10.1101/2023.05.29.542358.
5
Making the Most of your Electrons: Challenges and Opportunities in Characterizing Hybrid Interfaces with STEM.充分利用电子：用扫描透射电子显微镜表征混合界面的挑战与机遇
Mater Today (Kidlington). 2021 Nov;50:100-115. doi: 10.1016/j.mattod.2021.05.006. Epub 2021 Jun 19.
6
An Overview of Microcrystal Electron Diffraction (MicroED).微晶体电子衍射（MicroED）概述。
Annu Rev Biochem. 2021 Jun 20;90:431-450. doi: 10.1146/annurev-biochem-081720-020121.
7
Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.5 Å in cells.多粒子冷冻电镜重构技术 M 成功解析了细胞内 3.5Å 分辨率的核糖体-抗生素复合物。
Nat Methods. 2021 Feb;18(2):186-193. doi: 10.1038/s41592-020-01054-7. Epub 2021 Feb 4.
8
Direct transfer of electron microscopy samples to wetted carbon and graphene films via a support floatation block.通过支撑漂浮块将电子显微镜样品直接转移到湿润的碳和石墨烯薄膜上。
J Struct Biol. 2021 Mar;213(1):107677. doi: 10.1016/j.jsb.2020.107677. Epub 2020 Dec 8.
9
Advances in methods for atomic resolution macromolecular structure determination.原子分辨率大分子结构测定方法的进展。
F1000Res. 2020 Jul 2;9. doi: 10.12688/f1000research.25097.1. eCollection 2020.
10
Negative-Stain Transmission Electron Microscopy of Molecular Complexes for Image Analysis by 2D Class Averaging.用于二维分类平均图像分析的分子复合物负染色透射电子显微镜技术
Curr Protoc Microbiol. 2019 Sep;54(1):e90. doi: 10.1002/cpmc.90.
本文引用的文献
1
THE STRUCTURE OF ORF VIRUS.痘苗病毒的结构
Virology. 1964 Aug;23:461-72. doi: 10.1016/0042-6822(64)90230-2.
2
THE ANATOMY OF THE TOBACCO MOSAIC VIRUS.烟草花叶病毒的剖析
Virology. 1964 Mar;22:342-59. doi: 10.1016/0042-6822(64)90025-x.
3
Regulation of glutamine synthetase. XII. Electron microscopy of the enzyme from Escherichia coli.谷氨酰胺合成酶的调控。十二。大肠杆菌中该酶的电子显微镜观察
Biochemistry. 1968 Jun;7(6):2143-52. doi: 10.1021/bi00846a017.
4
A study of the structure of the T-layer of Bacillus brevis.短短芽孢杆菌T层结构的研究
J Supramol Struct. 1973;1(6):498-522. doi: 10.1002/jss.400010606.
5
Application of optical diffraction to helical structures in the bacteriophage tail.
Philos Trans R Soc Lond B Biol Sci. 1971 May 27;261(837):181-95. doi: 10.1098/rstb.1971.0049.
6
Molecular structure determination by electron microscopy of unstained crystalline specimens.通过电子显微镜对未染色晶体标本进行分子结构测定。
J Mol Biol. 1975 May 25;94(3):425-40. doi: 10.1016/0022-2836(75)90212-0.
7
Averaging of low exposure electron micrographs of non-periodic objects.非周期性物体低曝光电子显微照片的平均化处理。
Ultramicroscopy. 1975 Dec;1(2):159-62. doi: 10.1016/s0304-3991(75)80020-9.
8
Motif detection in quantum noise-limited electron micrographs by cross-correlation.通过互相关检测量子噪声限制电子显微照片中的基序
Ultramicroscopy. 1977 Apr;2(2-3):219-27. doi: 10.1016/s0304-3991(76)91385-1.
9
Signal to noise enhancement in dark field electron micrographs of vasopressin: filtering of arrays of images in reciprocal space.
J Microsc. 1977 Apr;109(3):259-68. doi: 10.1111/j.1365-2818.1977.tb01139.x.
10
Glutamine synthetase forms three- and seven-stranded helical cables.谷氨酰胺合成酶形成三股和七股螺旋束。
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3402-6. doi: 10.1073/pnas.72.9.3402.
Zotero 插件