Kessel M, Frank J, Goldfarb W
J Supramol Struct. 1980;14(4):405-22. doi: 10.1002/jss.400140402.
Averaged projections of individual glutamine synthetase molecules have been obtained by using electron microscopy and image processing. The methodology of correlation averaging under low dose conditions is described in detail. Because of their low signal-to-noise ratio, images made under low dose conditions cannot be directly interpreted in terms of high resolution features. Computer averaging of these images reveals a division of the subunit projection into two domains whose sizes agree with results of Lei et al [2] limited proteolysis experiments.
通过电子显微镜和图像处理获得了单个谷氨酰胺合成酶分子的平均投影。详细描述了低剂量条件下相关平均法的方法。由于低剂量条件下拍摄的图像信噪比低，无法直接根据高分辨率特征进行解释。对这些图像进行计算机平均处理后发现，亚基投影可分为两个结构域，其大小与Lei等人[2]的有限蛋白水解实验结果一致。
