Pediatric Surgery and Urology Department, Wroclaw Medical University, 52 M. Sklodowskiej - Curie ST, 50-369 Wroclaw, Poland.
Pediatric Surgery and Urology Department, Wroclaw Medical University, Wroclaw, Poland.
J Pediatr Surg. 2021 Feb;56(2):360-367. doi: 10.1016/j.jpedsurg.2020.05.046. Epub 2020 Jun 11.
Partial resection of the ovary carries a possible risk of fertility reduction. We studied the influence of open ovarian biopsy on ovarian reserve, including anti-Müllerian hormone and follicle-stimulating hormone serum level evaluation, in a prepubertal rat model.
Interventions - the initial surgery was unilateral ovarian biopsy (38 rats, group B1, B2) or unilateral ovarian biopsy and ovarian resection of the contralateral gonad (38 rats, group BR1, BR2). The second operation was bilateral ovarian resection and total resection of the remaining ovary. All rats had hormone serum levels evaluated. The control group had only a blood test taken and bilateral ovarian resection done at the second intervention (30 rats, group C1, C2). The collected tissue was examined estimating follicle count and anti-Müllerian hormone immunoexpression.
Anti-Müllerian hormone levels were significantly lower at the second intervention in the group BR2 but significantly higher in the group C2. Follicle-stimulating hormone levels were significantly higher in all but one group (BR2).
Biopsy itself might not reduce ovarian reserve if done properly but we should know its possible negative effects in the case of a single remaining ovary.
卵巢部分切除术可能会降低生育能力。我们研究了在未成年大鼠模型中，开放性卵巢活检对卵巢储备的影响，包括抗苗勒管激素和卵泡刺激素血清水平评估。
干预措施-初始手术为单侧卵巢活检（38 只大鼠，B1、B2 组）或单侧卵巢活检和对侧性腺卵巢切除术（38 只大鼠，BR1、BR2 组）。第二次手术为双侧卵巢切除术和剩余卵巢的完全切除术。所有大鼠均进行激素血清水平评估。对照组仅在第二次干预时进行血液检查和双侧卵巢切除术（30 只大鼠，C1、C2 组）。采集的组织进行检查，估计卵泡计数和抗苗勒管激素免疫表达。
BR2 组第二次干预时抗苗勒管激素水平显著降低，但 C2 组显著升高。除 BR2 组外，所有组的卵泡刺激素水平均显著升高。
如果操作正确，活检本身可能不会降低卵巢储备，但我们应该了解在单侧卵巢残留的情况下，它可能产生的负面影响。
