Department of Medicine, Division of Hematology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Department of Medicine, Division of Hematology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Trends Immunol. 2020 Aug;41(8):652-664. doi: 10.1016/j.it.2020.06.010. Epub 2020 Jul 9.
Cellular interactions in the tumor microenvironment (TME) significantly govern cancer progression and drug response. The efficacy of clinical immunotherapies has fostered an exponential interest in the tumor immune microenvironment, which in turn has engendered a pressing need for robust experimental systems modeling patient-specific tumor-immune interactions. Traditional 2D in vitro tumor immunotherapy models have reconstituted immortalized cancer cell lines with immune components, often from peripheral blood. However, newly developed 3D in vitro organoid culture methods now allow the routine culture of primary human tumor biopsies and increasingly incorporate immune components. Here, we present a viewpoint on recent advances, and propose translational applications of tumor organoids for immuno-oncology research, immunotherapy modeling, and precision medicine.
肿瘤微环境（TME）中的细胞相互作用显著影响着癌症的进展和药物反应。临床免疫疗法的疗效激发了人们对肿瘤免疫微环境的浓厚兴趣，这反过来又迫切需要强大的实验系统来模拟患者特异性的肿瘤免疫相互作用。传统的 2D 体外肿瘤免疫治疗模型中，通常使用外周血中的免疫成分来重建永生化的癌细胞系。然而，新开发的 3D 体外类器官培养方法现在可以常规培养原发性人类肿瘤活检组织，并越来越多地纳入免疫成分。在这里，我们对最近的进展提出了观点，并提出了肿瘤类器官在肿瘤免疫研究、免疫治疗建模和精准医学中的转化应用。
