Genetics and Plant Breeding Division, CSIR-Central Institute for Medicinal and Aromatic Plants, Lucknow, UP, 226015, India.
Bioinformatics Department, CSIR-National Botanical Research Institute, Lucknow, UP, 226001, India.
Appl Biochem Biotechnol. 2020 Nov;192(3):1076-1092. doi: 10.1007/s12010-020-03381-z. Epub 2020 Jul 13.
microRNAs are small non-coding RNA molecule that plays an important role in metabolism. Chrysopogon zizanioides (L.) Roberty is an important aromatic plant used in perfumery industries, soil, water conservation, and agricultural practices. In this study, the transcriptomic sequence of vetiver leaf and root was subjected to miRNA identification by the computational methods. miRNA identification was carried out using a homology-based method by C-mii software with several other online tools. A total of 80 miRNA were identified from both leaf and root sequences. Target identification was done by identified miRNA sets. A total of 25 and 31 miRNA families were identified in both leaf and root, respectively, with ten common families involve in different ontological function. miR169 and miR5021 regulate most of the target in leaf and root. In vetiver, many primary and secondary metabolism elements are regulated by miRNA as photo-system, transcription factor, terpenoid metabolism, etc. Here is the first in silico study revealing the specific miRNAs and their target genes for corresponding root and leaf tissues respectively in C. zizanioides.
miRNAs 是一种小的非编码 RNA 分子，在新陈代谢中起着重要作用。香根草（Chrysopogon zizanioides（L.）Roberty）是一种重要的香料植物，用于香水工业、土壤、水土保持和农业实践。在这项研究中，通过计算方法对香根草叶片和根系的转录组序列进行了 miRNA 鉴定。使用 C-mii 软件和其他几个在线工具，通过同源性方法进行 miRNA 鉴定。从叶片和根系序列中共鉴定出 80 个 miRNA。通过鉴定的 miRNA 集进行靶标鉴定。在叶片和根系中分别鉴定出 25 个和 31 个 miRNA 家族，其中 10 个常见家族涉及不同的本体功能。miR169 和 miR5021 调节叶片和根系中大多数靶基因。在香根草中，miRNA 调节许多初级和次级代谢元件，如光合作用系统、转录因子、萜类代谢等。这是首次在香根草中对相应的根和叶组织进行的特定 miRNA 及其靶基因的计算机研究。
