Biotechnology Division, Post Office CIMAP, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Appl Biochem Biotechnol. 2018 Aug;185(4):1145-1159. doi: 10.1007/s12010-018-2702-x. Epub 2018 Feb 24.
Withania somnifera, popularly known as Indian ginseng, is one of the most important medicinal plants. The plant is well studied in terms of its pharmaceutical activities and genes involved in biosynthetic pathways. However, not much is known about the regulatory mechanism of genes responsible for the production of secondary metabolites. The idea was to identify miRNA transcriptome responsible for the regulation of withanolide biosynthesis, specifically of root and leaf tissues individually. The transcriptome data of in vitro culture of root and leaf tissues of the plant was considered for miRNA identification. A total of 24 and 39 miRNA families were identified in root and leaf tissues, respectively. Out of these, 15 and 27 miRNA families have shown their involvement in different biological functions in root and leaf tissues, respectively. We report here, specific miRNAs and their corresponding target genes for corresponding root and leaf tissues. The target genes have also been analyzed for their role in withanolide metabolism. Endogenous root-miR5140, root-miR159, leaf-miR477, and leaf-miR530 were reported for regulation of withanolide biosynthesis.
睡茄，俗称印度人参，是最重要的药用植物之一。该植物在其药物活性及其参与生物合成途径的基因方面得到了很好的研究。然而，对于负责次生代谢产物生产的基因的调控机制知之甚少。我们的想法是鉴定负责调节白藜芦醇生物合成的 miRNA 转录组，特别是分别鉴定根和叶组织中的 miRNA 转录组。考虑了植物体外培养的根和叶组织的转录组数据来鉴定 miRNA。分别在根组织和叶组织中鉴定出了 24 个和 39 个 miRNA 家族。其中，有 15 个和 27 个 miRNA 家族分别显示出它们在根组织和叶组织中的不同生物学功能中的参与。我们在这里报告了特定的 miRNA 及其对应的根组织和叶组织的靶基因。还分析了靶基因在白藜芦醇代谢中的作用。内源性根-miR5140、根-miR159、叶-miR477 和叶-miR530 被报道参与白藜芦醇生物合成的调节。
