DiPette D J, Westlund K N, Holland O B
Department of Medicine, Marine Biomedical Institute, University of Texas Medical Branch, Galveston 77550.
Neurosci Lett. 1988 Dec 19;95(1-3):335-40. doi: 10.1016/0304-3940(88)90681-7.
This study was undertaken to determine if calcium status modulates calcitonin gene-related peptide (CGRP) neuronal content. In two separate experiments, young, growing rats and mature rats were placed on low, normal, and high calcium diets for four weeks. CGRP immunostaining was localized immunocytochemically in laminae I and II of the upper thoracic spinal cord in young rats and in the upper thoracic and lumbar spinal cord in mature rats. Low calcium intake decreased dorsal horn CGRP content in young, growing rats, while high calcium diet significantly increased CGRP content, as determined by computer-assisted image processing, in both young and adult rats. A significant positive correlation was found between the serum ionized calcium and CGRP content in laminae I and II. Thus, calcium balance appears to modulate neuronal CGRP content.
本研究旨在确定钙状态是否会调节降钙素基因相关肽（CGRP）的神经元含量。在两个独立的实验中，将幼年生长大鼠和成年大鼠分别置于低钙、正常钙和高钙饮食中四周。通过免疫细胞化学方法，在幼年大鼠上胸段脊髓的I层和II层以及成年大鼠的上胸段和腰段脊髓中对CGRP进行免疫染色定位。通过计算机辅助图像处理确定，低钙摄入降低了幼年生长大鼠背角CGRP的含量，而高钙饮食则显著增加了幼年和成年大鼠的CGRP含量。在血清离子钙与I层和II层CGRP含量之间发现了显著的正相关。因此，钙平衡似乎会调节神经元CGRP的含量。
