Jiangsu Institute of Poultry Sciences, Supervision, Inspection and Testing Centre for Poultry Quality (Yangzhou), Ministry of Agriculture, Yangzhou 225125, China.
Jiangsu Key Lab of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225003, China.
FEMS Microbiol Lett. 2020 Jul 1;367(14). doi: 10.1093/femsle/fnaa115.
Campylobacter is a leading causative pathogen of acute bacterial gastroenteritis among humans. Contaminated chicken products are regarded as major sources of human infection. The flagellar capping protein (FliD), which plays important roles in colonization and adhesion to the mucosal surface of chicken ceca, is conserved among Campylobacter jejuni strains. In this study, the recombinant C. jejuni FliD protein was expressed, purified and used as a coated protein to examine the prevalence of C. jejuni antibodies in chickens. The anti-FliD antibody was prevalent among chicken serum samples taken from different farms in the diverse regions of Jiangsu province by using enzyme-linked immunosorbent assay. The Campylobacter antibody was present in culture-negative chickens. No strong dose-response relationships were observed between serum FliD antibody levels and Campylobacter cultural status. These results provide a basis for further evaluating FliD as a vaccine candidate for broiler chickens or for examining host-C. jejuni interactions, with implications for improving food safety.
空肠弯曲菌是人类急性细菌性肠胃炎的主要病原体之一。受污染的鸡肉产品被认为是人类感染的主要来源。鞭毛顶帽蛋白（FliD）在定植和黏附鸡盲肠黏膜表面方面发挥着重要作用，在空肠弯曲菌菌株中具有保守性。在本研究中，表达、纯化了重组空肠弯曲菌 FliD 蛋白，并将其作为包被蛋白，用于检测鸡群中空肠弯曲菌抗体的流行情况。通过酶联免疫吸附试验，在江苏省不同地区不同农场采集的鸡血清样本中，抗 FliD 抗体普遍存在。在培养阴性的鸡中也存在弯曲菌抗体。血清 FliD 抗体水平与弯曲菌培养状态之间没有明显的剂量反应关系。这些结果为进一步评估 FliD 作为肉鸡疫苗候选物或研究宿主-空肠弯曲菌相互作用提供了依据，这对于提高食品安全具有重要意义。
