Ryu A-Reum, Kim Yong-Wan, Lee Mi-Young
Department of Medical Biotechnology, Soonchunhyang University, 22 Soonchunhyang-ro, Asan-si, 31538, Republic of Korea.
Daegu Cancer Center, Research and Development Unit, DongSung Bio-Pharmaceuticals Co. Ltd., 8, Cheombok-ro, Daegu-si, 41061, Republic of Korea.
Photodiagnosis Photodyn Ther. 2020 Sep;31:101917. doi: 10.1016/j.pdpdt.2020.101917. Epub 2020 Jul 10.
Chlorin e6-mediated photodynamic therapy (Ce6-mediated PDT) is a therapeutic modality that consists of a photosensitizer, source of light at a suitable wavelength, and molecular oxygen. PDT has been widely used for the treatment of cancers and non-neoplastic disorders. However, information on the inhibitory activity of Ce6-mediated PDT on adipogenesis and lipogenesis in 3T3-L1 cells is not available to date.
MTT assay, Oil Red O staining, and Nile red staining were used to investigate lipid accumulation in 3T3-L1 cells to clarify adipocyte differentiation. The expression levels of various molecular signals associated with adipogenesis and lipogenesis were examined by RT-PCR and western blot analysis.
Adipocyte differentiation and lipid accumulation were reduced by Ce6-mediated PDT in 3T3-L1 cells. Inhibition of the expression of PPAR-γ, C/EBPα, β, δ, SREBP-1, FAS, and LPL and the activation of AMPK, revealed concise modulation of adipogenic and lipogenic molecules by Ce6-mediated PDT. The anti-adipogenic effect of Ce6-mediated PDT was reversed by AMPKα siRNA.
Ce6-mediated PDT inhibits adipocyte differentiation and lipogenesis via regulating AMPK. Ce6-mediated PDT might serve as a potential therapy for the treatment of obesity and obesity-associated diseases.
二氢卟吩e6介导的光动力疗法（Ce6介导的光动力疗法）是一种治疗方式，由一种光敏剂、合适波长的光源和分子氧组成。光动力疗法已广泛用于癌症和非肿瘤性疾病的治疗。然而，迄今为止，关于Ce6介导的光动力疗法对3T3-L1细胞脂肪生成和脂质生成的抑制活性尚无相关信息。
采用MTT法、油红O染色和尼罗红染色来研究3T3-L1细胞中的脂质积累，以阐明脂肪细胞分化情况。通过逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹分析检测与脂肪生成和脂质生成相关的各种分子信号的表达水平。
Ce6介导的光动力疗法可减少3T3-L1细胞中的脂肪细胞分化和脂质积累。抑制过氧化物酶体增殖物激活受体γ（PPAR-γ）、CCAAT/增强子结合蛋白α（C/EBPα）、β、δ、固醇调节元件结合蛋白1（SREBP-1）、脂肪酸合成酶（FAS）和脂蛋白脂肪酶（LPL）的表达以及激活腺苷酸活化蛋白激酶（AMPK），揭示了Ce6介导的光动力疗法对脂肪生成和脂质生成分子的精确调节作用。Ce6介导的光动力疗法的抗脂肪生成作用被AMPKα小干扰RNA（siRNA）逆转。
Ce6介导的光动力疗法通过调节AMPK抑制脂肪细胞分化和脂质生成。Ce6介导的光动力疗法可能成为治疗肥胖症及肥胖相关疾病的一种潜在疗法。
