Paganelli R, Quinti I, D'Offizi G P, Papetti C, Nisini R, Aiuti F
Department of Allergy and Clinical Immunology, University La Sapienza, Rome, Italy.
J Clin Lab Immunol. 1988 Jul;26(3):153-7.
We isolated a human IgG anti IgE autoantibody by affinity chromatography on a myelomatous IgE (N.D.) coated Sepharose column, from the serum of a patient with atopic eczema, and probed its ability to inhibit IgE recognition by heteroantisera to IgE used in commonly available assays for serum total and specific IgE determinations (Paper radio-immunosorbent test--PRIST and Phadezym radioallergosorbent test--RAST, both from Pharmacia, Uppsala--Sweden). Our results indicate that IgG anti IgE decrease total IgE results by 10-90% according to its weight ratio to IgE molecules present in samples devoid of endogeneous anti-IgE. Sera with high content of specific IgE to house dust mite (RAST class 3 or 4) are less affected by this autoantibody, but RAST class 2 may be significantly lowered when purified IgG anti IgE is added. These data imply that anti IgE can affect both total and specific IgE determinations.
我们通过在包被有骨髓瘤IgE（N.D.）的琼脂糖柱上进行亲和层析，从一名特应性皮炎患者的血清中分离出一种人IgG抗IgE自身抗体，并检测了其抑制用于血清总IgE和特异性IgE测定的常用检测方法中使用的IgE异源抗血清识别IgE的能力（纸放射免疫吸附试验——PRIST和法德齐姆放射变应原吸附试验——RAST，均来自瑞典乌普萨拉的法玛西亚公司）。我们的结果表明，IgG抗IgE根据其与不含内源性抗IgE的样品中存在的IgE分子的重量比将总IgE结果降低10%至90%。对屋尘螨特异性IgE含量高的血清（RAST 3级或4级）受这种自身抗体的影响较小，但当加入纯化的IgG抗IgE时，RAST 2级可能会显著降低。这些数据表明抗IgE可影响总IgE和特异性IgE的测定。
