Pestana Ana, Batista Rui, Celestino Ricardo, Canberk Sule, Sobrinho-Simões Manuel, Soares Paula
Institute of Molecular Pathology and Immunology of the University of Porto (Ipatimup), 4200-135 Porto, Portugal.
i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal.
Cancers (Basel). 2020 Jul 9;12(7):1846. doi: 10.3390/cancers12071846.
The presence of promoter (p) mutations in thyroid cancer have been associated with worse prognosis features, whereas the extent and meaning of the expression and activation of in thyroid tumours is still largely unknown. We analysed frozen samples from a series of benign and malignant thyroid tumours, displaying non-aggressive features and low mutational burden in order to evaluate the presence of p mutations and mRNA expression in these settings. In this series, p mutations were found in 2%, only in malignant cases, in larger cancers, and from older patients. mRNA expression was detected in both benign and malignant tumours, with increased frequencies in the malignant tumours with aggressive histotypes, larger tumours, and from older patients. In benign tumours, mRNA expression was found in 17% of the follicular thyroid adenoma (FTA) with increased levels of expression in smaller tumours and associated with the presence of thyroiditis. p mutations and mRNA expression are correlated with worse prognosis features in malignant thyroid tumours, whereas mRNA expression in the benign tumours is associated with the presence of thyroiditis.
甲状腺癌中启动子（p）突变的存在与较差的预后特征相关，而甲状腺肿瘤中其表达和激活的程度及意义仍 largely unknown。我们分析了一系列具有非侵袭性特征和低突变负荷的良性和恶性甲状腺肿瘤的冷冻样本，以评估这些情况下p突变的存在及mRNA表达。在该系列中，p突变仅在恶性病例、较大的癌症以及老年患者中被发现，占2%。在良性和恶性肿瘤中均检测到mRNA表达，在具有侵袭性组织学类型的恶性肿瘤、较大肿瘤以及老年患者中频率增加。在良性肿瘤中，17%的滤泡性甲状腺腺瘤（FTA）中发现mRNA表达，在较小肿瘤中表达水平升高且与甲状腺炎的存在相关。p突变和mRNA表达与恶性甲状腺肿瘤较差的预后特征相关，而良性肿瘤中的mRNA表达与甲状腺炎的存在相关。
