Chemical Engineering Discipline, School of Engineering, Monash University Malaysia, Bandar Sunway, Selangor 47500, Malaysia.
World Pranic Healing Foundation, India Research Center, 4th Main, Saraswathipuram, Mysore, Karnataka 570009, India.
Biomolecules. 2020 Jul 9;10(7):1019. doi: 10.3390/biom10071019.
Agro-industrial waste is a largely untapped natural resource of bioactive compounds including carotenoids and pectin. However, conventional solvent extraction involves the excessive use of organic solvents, costly equipment, and tedious operation. These limitations of conventional extraction methods could be prospectively overcome by the carotenoid-pectin hydrocolloidal complexation. The complexation of lycopene and pectin was efficiently promoted in an aqueous environment, resulting in the colloidal complexes that can be subsequently recovered by sedimentation or centrifugation. In this study, the potential of carotenoid-pectin complexation on tomato pomace containing carotenoids and pectin was evaluated. Tomato pomace is a rich source of lycopene, β-carotene as well as pectin, making it suitable as the raw material for the carotenoid extraction. The extraction of carotenoid and pectin from tomato pomace was optimized using response surface methodology. The maximum recovery was 9.43 mg carotenoid fractions/100 g tomato pomace, while the purity of carotenoid-rich fractions was 92%. The antioxidant capacity of carotenoids extracted from the complexation method was found to be higher than that from the solvent extraction method. Moreover, extraction yield and antioxidant capacity of carotenoid obtained from the carotenoid-pectin complexation were comparable to that from solvent extraction. The carotenoid-pectin complexation is a promising green approach to valorize agro by-products for the extraction of valuable carotenoids.
农业工业废物是生物活性化合物（包括类胡萝卜素和果胶）的一种未被充分开发的自然资源。然而，传统的溶剂萃取法涉及有机溶剂的大量使用、昂贵的设备和繁琐的操作。这些传统提取方法的局限性可以通过类胡萝卜素-果胶水溶胶复合作用来克服。在水相环境中，番茄渣中类胡萝卜素和果胶的复合作用得到了有效的促进，形成了可以通过沉降或离心回收的胶体复合物。在这项研究中，评估了类胡萝卜素-果胶复合作用对含有类胡萝卜素和果胶的番茄渣的潜力。番茄渣是番茄红素、β-胡萝卜素和果胶的丰富来源，使其成为提取类胡萝卜素的理想原料。使用响应面法对番茄渣中类胡萝卜素和果胶的提取进行了优化。最大的类胡萝卜素回收率为 9.43mg 类胡萝卜素/100g 番茄渣，而富类胡萝卜素部分的纯度为 92%。从复合方法中提取的类胡萝卜素的抗氧化能力被发现高于溶剂提取方法。此外，从类胡萝卜素-果胶复合作用中提取的类胡萝卜素的提取率和抗氧化能力与溶剂提取相当。类胡萝卜素-果胶复合作用是一种有前途的绿色方法，可以利用农业副产品来提取有价值的类胡萝卜素。
