Koide J, Takeuchi T, Hosono O, Takano M, Abe T
Second Department of Internal Medicine, Saitama Medical School, Japan.
Scand J Immunol. 1988 Dec;28(6):687-96. doi: 10.1111/j.1365-3083.1988.tb01502.x.
We produced monoclonal anti-idiotypic antibody against immunoaffinity purified anti-U1-ribonucleoprotein (RNP) antibody from a patient (K.T.), by the cell fusion procedure. The specificity of monoclonal anti-idiotypic antibody (IgG1, kappa) was determined by inhibition studies. With the monoclonal anti-idiotypic antibody, cross-reactive idiotypes on anti-U1-RNP antibodies from unrelated patients with anti-U1-RNP antibody was detected in 57% of the samples. The anti-idiotypic antibody specifically suppressed the in vitro production of anti-U1-RNP antibody by lymphocytes from the patient K.T., and unrelated patients with a cross-reactive idiotype, in whom idiotype-reactive T cells were demonstrated. The results indicate that anti-idiotypic antibody may modulate the regulation of in vitro anti-U1-RNP antibody production.
我们通过细胞融合程序，制备了针对一名患者（K.T.）经免疫亲和纯化的抗U1核糖核蛋白（RNP）抗体的单克隆抗独特型抗体。通过抑制研究确定了单克隆抗独特型抗体（IgG1，κ）的特异性。使用该单克隆抗独特型抗体，在57%的样本中检测到了来自无关抗U1-RNP抗体患者的抗U1-RNP抗体上的交叉反应性独特型。该抗独特型抗体特异性抑制了患者K.T.以及具有交叉反应性独特型且已证明存在独特型反应性T细胞的无关患者的淋巴细胞在体外产生抗U1-RNP抗体。结果表明抗独特型抗体可能调节体外抗U1-RNP抗体产生的调节。
