Akimoto Y, Obinata A, Endo H, Hirano H
Department of Anatomy, Kyorin University School of Medicine, Tokyo, Japan.
Cell Tissue Res. 1988;254(3):481-5. doi: 10.1007/BF00226497.
The effect of epidermal growth factor (EGF) on the basement membrane structure of chick embryonic skin cultured in a chemically defined medium (BGJb) containing 20 mM hydrocortisone, and EGF at 10, 50, or 100 ng/ml supplemented with 5% delipidized fetal calf serum, was examined by electron microscopy. During development of the epidermis in vitro, EGF (100 ng/ml) caused striking changes to occur in the basement membrane structure and in the keratinization process. The basement membrane frequently became discontinuous with many gaps apparent in section, and occasionally became folded following detachment from the basal surface of the epidermis and protruded into the underlying dermis. In the basal and intermediate cells of EGF-treated epidermis, tonofilament bundles were decreased in number, while desmosomes and hemidesmosomes revealed no significant changes in morphology.
通过电子显微镜检查了表皮生长因子（EGF）对在含有20 mM氢化可的松的化学限定培养基（BGJb）中培养的鸡胚皮肤基底膜结构的影响，该培养基添加了5%脱脂胎牛血清，并补充有10、50或100 ng/ml的EGF。在体外表皮发育过程中，EGF（100 ng/ml）导致基底膜结构和角质化过程发生显著变化。基底膜常常变得不连续，切片中可见许多间隙，偶尔在从表皮基底表面脱离后发生折叠并突入下方的真皮。在经EGF处理的表皮的基底细胞和中间细胞中，张力丝束数量减少，而桥粒和半桥粒在形态上没有显著变化。
