黄独地上块茎贮藏蛋白的物理化学、酶学及分子特性研究
Physicochemical, enzymatic and molecular characterisation of the storage protein of aerial tuber, Dioscorea bulbifera Linn.
作者信息
Osukoya Olukemi Adetutu, Kuku Adenike
机构信息
Department of Chemical Sciences, Afe Babalola University, Ado Ekiti, Nigeria.
Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, Nigeria.
出版信息
J Genet Eng Biotechnol. 2020 Jul 13;18(1):29. doi: 10.1186/s43141-020-00040-y.
BACKGROUND
The storage protein of the aerial tuber of Dioscorea bulbifera was purified and its physicochemical, enzymatic and molecular properties determined with a view to comparing its functionality and genetic relatedness with other storage proteins.
RESULTS
The purified protein had molecular weight of 21 kDa. The protein showed carbonic anhydrase, trypsin inhibitory, dehydroascorbate reductase and monodehydroascorbate reductase activities. Amplifications with polymerase chain reactions resulted in the detection of two genes encoding the storage protein. The deduced amino acid sequence of the shorter and larger genes had homologies with the storage proteins of members of the Dioscorea family.
CONCLUSION
The study concluded that the storage protein of the aerial tuber of D. bulbifera had similar properties with those of other Dioscorea species and may be suitable for development as functional food.
背景
对黄独气生块茎的贮藏蛋白进行了纯化，并测定了其物理化学、酶学和分子特性，以便将其功能和遗传相关性与其他贮藏蛋白进行比较。
结果
纯化后的蛋白分子量为21 kDa。该蛋白具有碳酸酐酶、胰蛋白酶抑制、脱氢抗坏血酸还原酶和单脱氢抗坏血酸还原酶活性。通过聚合酶链反应扩增，检测到两个编码该贮藏蛋白的基因。较短和较长基因推导的氨基酸序列与薯蓣科成员的贮藏蛋白具有同源性。
结论
该研究得出结论，黄独气生块茎的贮藏蛋白与其他薯蓣属物种的贮藏蛋白具有相似特性，可能适合开发为功能性食品。
Zotero 插件