Shimizu T, Harada T, Majima Y, Sakakura Y
Department of Otorhinolaryngology, Mie University School of Medicine, Tsu Japan.
Int J Pediatr Otorhinolaryngol. 1988 Dec;16(3):211-7. doi: 10.1016/0165-5876(88)90032-8.
Bactericidal activity of middle ear effusion (MEE) from children with otitis media with effusion (OME) on one strain of non-typable Haemophilus influenzae was measured and was compared with that of normal human serum. H. influenzae culture negative MEEs had a bactericidal activity on one strain of non-typable H. influenzae (P less than 0.01), and the activity of serous effusions was significantly stronger than that of mucoid ones (P less than 0.01). The bactericidal activity of 20% pooled MEE was almost equal to that of 4% normal human serum. Heat treatment (56 degrees C, 30 min) abolished this activity of both pooled MEE and normal human serum. Addition of fresh guinea pig serum (GPS) as a complement source to the heat-treated pooled MEE restored this activity, while its addition to the heat-treated normal human serum failed to do so. These results suggest that complement factor is important in the bactericidal activity of MEE.
对患有分泌性中耳炎（OME）儿童的中耳积液（MEE）针对一株不可分型流感嗜血杆菌的杀菌活性进行了测定，并与正常人血清的杀菌活性进行了比较。流感嗜血杆菌培养阴性的MEE对一株不可分型流感嗜血杆菌具有杀菌活性（P<0.01），浆液性积液的活性明显强于黏液性积液（P<0.01）。20%混合MEE的杀菌活性几乎等同于4%正常人血清的杀菌活性。热处理（56℃，30分钟）消除了混合MEE和正常人血清的这种活性。向热处理后的混合MEE中添加新鲜豚鼠血清（GPS）作为补体来源可恢复该活性，而向热处理后的正常人血清中添加则无法恢复。这些结果表明补体因子在MEE的杀菌活性中很重要。
