蛙皮素及其类似物可抑制白细胞介素-2诱导的CTLL-2细胞增殖。

Bombesin and its analogues inhibit interleukin-2-induced proliferation of CTLL-2 cells.

作者信息

Fink R, Ehrhardt R, Dancygier H

机构信息

II. Medizinische Klinik und Poliklinik, Technischen Universität München, F.R.G.

出版信息

Regul Pept. 1988 Dec;23(3):323-30. doi: 10.1016/0167-0115(88)90233-9.

DOI:10.1016/0167-0115(88)90233-9

PMID:3266344

Abstract

Specifically interleukin-2 (IL-2)-dependent CTLL-2 cells were incubated in short term culture in the presence of IL-2 together with bombesin and two analogues, [Lys3]bombesin and [Tyr4]bombesin in different concentrations. Cell proliferation, determined by [3H]thymidine incorporation was significantly inhibited by 35.6 +/- 5%, 39.0 +/- 5.6% and 57.0 +/- 11% (mean +/- S.E.M. of 3 independent experiments). A typically U-shaped dose-response relationship was observed, showing a maximum effect between 10(-12) and 10(-10) M. Our data support the hypothesis that this effect is mediated by a specific receptor for bombesin and closely related peptides on CTLL-2 cells. As IL-2 plays a critical role in the clonal expansion of activated lymphocytes, antagonism of the effect of IL-2 is of high biological significance.

摘要

具体而言，依赖白细胞介素-2（IL-2）的CTLL-2细胞在短期培养中与铃蟾肽及两种类似物[Lys3]铃蟾肽和[Tyr4]铃蟾肽一起，在不同浓度的IL-2存在下进行孵育。通过[3H]胸腺嘧啶核苷掺入法测定的细胞增殖受到显著抑制，分别为35.6 +/- 5%、39.0 +/- 5.6%和57.0 +/- 11%（3次独立实验的平均值 +/- 标准误）。观察到典型的U形剂量反应关系，在10(-12)至10(-10) M之间呈现最大效应。我们的数据支持这样的假说，即这种效应是由CTLL-2细胞上铃蟾肽及密切相关肽的特异性受体介导的。由于IL-2在活化淋巴细胞的克隆扩增中起关键作用，对IL-2效应的拮抗具有高度生物学意义。