Xu Haitao, Qu Yiming
Department of Orthopedics, The Yongchuan Hospital of Chongqing Medical University, Chongqing, China.
Medicine (Baltimore). 2020 Jul 10;99(28):e19942. doi: 10.1097/MD.0000000000019942.
The present study was performed to statistically explore the effect of anthrax toxin receptor 2 (ANTXR2) polymorphism rs4333130 on individual susceptibility to ankylosing spondylitis (AS) using the method of meta-analysis.
All of the eligible reports were retrieved from well-known electronic databases. The strength of the association between ANTXR2 polymorphism rs4333130 and the susceptibility to AS was evaluated using pooled odds ratios (ORs) with 95% confidence intervals (95% CIs). In addition, subgroup analysis was also performed on the basis of ethnicity to further explore specific correlation between our studied polymorphism and the disease risk. Inter-study heterogeneity was detected with Q test, and P < .05 was considered statistically significant. Sensitivity analysis was implemented through removing each of eligible studies and then recalculating overall effects to test the reliability of final estimates. Publication bias among included studies was inspected with both Begg funnel plot and Egger regression test.
A total of 6 eligible papers were finally incorporated into the present meta-analysis. In total analysis, ANTXR2 polymorphism rs4333130 was significantly related to decreased risk of AS under CC versus TT, CC + TC versus TT, CC versus TT + TC, C versus T and TC versus TT contrasts (OR = 0.35, 95% CI = 0.20-0.64; OR = 0.81, 95% CI = 0.69-0.95; OR = 0.38, 95% CI = 0.21-0.68; OR = 0.89, 95% CI = 0.84-0.95; OR = 0.84, 95% CI = 0.72-0.99). Moreover, a similar effect was also observed in Asian and Caucasian subgroups under corresponding genetic models after stratification analysis based on ethnicity.
ANTXR2 polymorphism rs4333130 may function as a protective factor against AS incidence.
本研究采用荟萃分析方法，旨在从统计学角度探讨炭疽毒素受体2（ANTXR2）基因多态性rs4333130对个体患强直性脊柱炎（AS）易感性的影响。
从知名电子数据库中检索所有符合条件的报告。使用合并比值比（OR）及95%置信区间（95%CI）评估ANTXR2基因多态性rs4333130与AS易感性之间的关联强度。此外，还基于种族进行亚组分析，以进一步探究所研究的基因多态性与疾病风险之间的具体相关性。采用Q检验检测研究间异质性，P<0.05被认为具有统计学意义。通过逐一剔除符合条件的研究，然后重新计算总体效应来进行敏感性分析，以检验最终估计值的可靠性。采用Begg漏斗图和Egger回归检验检查纳入研究中的发表偏倚。
最终共有6篇符合条件的论文纳入本荟萃分析。在总体分析中，在CC与TT、CC+TC与TT、CC与TT+TC、C与T以及TC与TT对比中，ANTXR2基因多态性rs4333130与AS风险降低显著相关（OR=0.35，95%CI=0.20-0.64；OR=0.81，95%CI=0.69-0.95；OR=0.38，95%CI=0.21-0.68；OR=0.89，95%CI=0.84-0.95；OR=0.84，95%CI=0.72-0.99）。此外，基于种族进行分层分析后，在相应遗传模型下的亚洲和白种人亚组中也观察到了类似效应。
ANTXR2基因多态性rs4333130可能是AS发病的一个保护因素。
