Bubenik J, Voitenok N N, Kieler J, Prassolov V S, Chumakov P M, Bubenikova D, Simova J, Jandlova T
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Immunol Lett. 1988 Dec;19(4):279-82. doi: 10.1016/0165-2478(88)90155-1.
We have prepared a retroviral expression construct, pPS-IL-2, in which human IL-2 cDNA has been inserted into the polylinker region, and have used the retroviral vector to introduce the functional IL-2 gene into a fibroblast cell line, RAT-1. Peritumoral administration of IL-2-producing RAT-1 cells into congenitally athymic (nu/nu) mice carrying subcutaneous transplants of human carcinoma cells inhibited the growth of the human tumour xenografts.
我们制备了一种逆转录病毒表达构建体pPS-IL-2，其中人IL-2 cDNA已插入多克隆位点区域，并使用该逆转录病毒载体将功能性IL-2基因导入成纤维细胞系RAT-1。将产生IL-2的RAT-1细胞瘤周注射到携带人癌细胞皮下移植瘤的先天性无胸腺（nu/nu）小鼠体内，可抑制人肿瘤异种移植瘤的生长。
