Tomita Y, Dustoor M M, Cathcart M K
Department of Immunology and Cancer, Research Institute, Cleveland Clinic Foundation, OH 44106.
Immunopharmacology. 1988 Nov-Dec;16(3):199-205. doi: 10.1016/0162-3109(88)90008-2.
A human thymus cell hybridoma secretes an immunosuppressive lymphokine, referred to as hybridoma suppressor factor (HSF). This factor suppresses polyclonal immunoglobulin (Ig) production as well as Interleukin-2 (IL-2) production in pokeweed mitogen (PWM)-stimulated peripheral blood mononuclear cells (PBMC). Similar suppression of Ig and IL-2 production was observed in reconstituted cultures of CD4+ cells and non-T cells. Here we analysed, further, the mechanism of HSF-mediated suppression of Ig and IL-2 production. We demonstrated that HSF inhibited PWM-induced IL-2 production by CD4+ cells but not by CD8+ cells and its suppressive activity on Ig production was totally abrogated by preabsorption with CD4+ cells, but not by CD8+ cells. These results indicate a subset specific action of HSF.
一种人胸腺细胞杂交瘤分泌一种免疫抑制性淋巴因子，称为杂交瘤抑制因子（HSF）。该因子可抑制在商陆丝裂原（PWM）刺激的外周血单个核细胞（PBMC）中多克隆免疫球蛋白（Ig）的产生以及白细胞介素-2（IL-2）的产生。在CD4+细胞和非T细胞的重组培养物中也观察到了对Ig和IL-2产生的类似抑制作用。在此，我们进一步分析了HSF介导的对Ig和IL-2产生的抑制机制。我们证明，HSF抑制PWM诱导的CD4+细胞产生IL-2，但不抑制CD8+细胞产生IL-2，并且其对Ig产生的抑制活性在与CD4+细胞预吸收后完全消除，但与CD8+细胞预吸收则不会消除。这些结果表明HSF具有亚群特异性作用。
