Yahaya Muhammad Sanusi, Salisi Mohd Shahrom, Md Isa Nur Mahiza, Meng Goh Yong, Haron Abdwahid
Department of Clinical Studies, Faculty of Veterinary Medicine Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
Department of Pathology and Microbiology, Faculty of Veterinary Medicine Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.
Future Sci OA. 2020 Jun 2;6(6):FSO580. doi: 10.2144/fsoa-2020-0037.
A number of factors are known to reduce fertility rate in animals and one of the important categories of such factors is chromosome anomalies. They can occur with or without causing phenotypic abnormalities on animals; in some cases, they may directly affect meiosis, gametogenesis and the viability of conceptus. In many instances, balanced structural rearrangements can be transmitted to offspring, affecting fertility in subsequent generations.
This work investigated the occurrence of chromosome aberrations in , and raised in a nucleus deer farm in Malaysia with a history of declining fertility of unknown origin.
MATERIALS & METHODS: Blood samples were collected from 60 animals through venipuncture, cultured for 72 h and arrested at metaphase. SmartType and Ideokar software were used to karyotype the chromosomes.
We found 15 out of the 60 animals screened from both sexes harbor some form of chromosome aberration. Chromosomal aberrations exist at the rate of 25% and may not be unconnected with the observed reduced fertility on the farm. Further investigations should be carried out, especially on the offspring of the studied animals to transmission of these aberrations. The animals that are confirmed to transmit the chromosomal aberrations should be culled to arrest the propagation of their abnormalities.
已知多种因素会降低动物的生育率，其中重要的一类因素是染色体异常。它们可能在不引起动物表型异常的情况下出现；在某些情况下，它们可能直接影响减数分裂、配子发生和胚胎的活力。在许多情况下，平衡的结构重排可以传递给后代，影响后代的生育能力。
本研究调查了马来西亚一个核鹿场中 、 和 的染色体畸变情况，该鹿场有生育力下降且原因不明的历史。
通过静脉穿刺从60只动物采集血液样本，培养72小时并阻断在中期。使用SmartType和Ideokar软件对染色体进行核型分析。
我们发现从两性中筛选的60只动物中有15只存在某种形式的染色体畸变。染色体畸变率为25%，这可能与该鹿场观察到的生育力下降有关。应进一步开展调查，特别是对所研究动物的后代进行调查，以了解这些畸变的传递情况。应淘汰被证实会传递染色体畸变的动物，以阻止其异常情况的传播。
