两种渔药对鲫鱼（Carassius auratus gibelio）GAD 和 GABA-T mRNA 表达的影响。
Effects of two kinds of fishery drugs on the expressions of GAD and GABA-T mRNA in crucian carp (Carassius auratus gibelio).
机构信息
Department of Aquaculture, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China.
National Center for Aquatic Pathogen Collection, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
出版信息
Fish Physiol Biochem. 2020 Dec;46(6):1965-1973. doi: 10.1007/s10695-020-00847-z. Epub 2020 Jul 15.
The objective of this study was to investigate the effects of difloxacin (DIF) and avermectin (AVM) on glutamate decarboxylase (GAD) and GABA-transaminase (GABA-T) in different tissues of crucian carp (Carassius auratus gibelio). After the treatments of DIF and AVM, the mRNA expressions of GAD and GABA-T in different tissues were detected by quantitative real-time PCR (qPCR). The results showed that the mRNA expressions of GAD, GAD, and GABA-T in the telencephalon (Tel), mesencephalon (Mes), cerebella (Cer), and medulla oblongata (Med) were downregulated significantly with the safe dose (SD, 20 mg/kg) of DIF (P < 0.05 or P < 0.01). While the expressions of GAD and GAD in the kidney at 12 h had strikingly upregulated to 13.81 ± 1.06** and 150.67 ± 12.85** times. Treated with the lethal dose of 50% (LD, 2840 mg/kg b. W.) of DIF, the mRNA expressions of GAD, GAD, and GABA-T in all tissues were increased significantly (P < 0.01). The results of AVM group showed that the mRNA expressions of GAD, GAD, and GABA-T both in the central and peripheral tissues were all remarkably downregulated at the safe concentration (SC, 0.0039 mg/L) and the lethal concentration of 50% (LC, 0.039 mg/L), except for the mRNA inhibitions of GAD, GAD, and GABA-T in the muscle at 2 h which sharply downregulated to 0.20 ± 0.02 × 10, 0.57 ± 0.06 × 10 and 0.44 ± 0.02 × 10, respectively (P < 0.01).
本研究旨在探讨氧氟沙星（DIF）和阿维菌素（AVM）对鲫鱼（Carassius auratus gibelio）不同组织中谷氨酸脱羧酶（GAD）和 GABA-转氨酶（GABA-T）的影响。经 DIF 和 AVM 处理后，采用实时荧光定量 PCR（qPCR）检测不同组织中 GAD 和 GABA-T 的 mRNA 表达。结果表明，安全剂量（SD，20mg/kg）的 DIF 可显著下调大脑（Tel）、中脑（Mes）、小脑（Cer）和延髓（Med）中 GAD、GAD 和 GABA-T 的 mRNA 表达（P<0.05 或 P<0.01）。而在 12h 时，肾脏中 GAD 和 GAD 的表达则显著上调至 13.81±1.06和 150.67±12.85倍。用 50%致死剂量（LD，2840mg/kg b.w.）的 DIF 处理后，所有组织中 GAD、GAD 和 GABA-T 的 mRNA 表达均显著增加（P<0.01）。AVM 组结果表明，在安全浓度（SC，0.0039mg/L）和 50%致死浓度（LC，0.039mg/L）下，中枢和外周组织中 GAD、GAD 和 GABA-T 的 mRNA 表达均显著下调，除了肌肉中 GAD、GAD 和 GABA-T 的 mRNA 抑制在 2h 时急剧下调至 0.20±0.02×10、0.57±0.06×10 和 0.44±0.02×10（P<0.01）。
Zotero 插件