Post-Graduation Program in Conservation of Natural Resources in Cerrado, Goiano Federal Institute of Education, Science and Technology - Urutaí Campus, Urutaí, GO, Brazil.
Laboratório de Pesquisas Biológicas, Instituto Federal Goiano - Campus Urutaí, Rodovia Geraldo Silva Nascimento, 2.5 km, Zona Rural, Urutaí, GO, 75790-000, Brazil.
Environ Sci Pollut Res Int. 2018 Jan;25(3):2450-2456. doi: 10.1007/s11356-017-0677-8. Epub 2017 Nov 10.
Although previous studies have already confirmed the toxicological potential of abamectin (ABA) in different experimental models (from invertebrates to vertebrates), almost nothing is known about the impacts the exposure to this pesticide can cause on birds. Thus, the aim of our study is to investigate the cytotoxic effects on the erythrocytes of female Japanese quails (Coturnix japonica) exposed to low abamectin concentrations. In order to do so, three experimental groups were proposed: "control," composed of quails exposed to abamectin-free drinking water; "ABA 1% median lethal dose (LD)," comprising birds exposed to water containing 15.5 mg a.i./L of abamectin (via commercial formulation Kraft® 36EC), and "ABA 10% LD," composed by birds exposed to water containing 155.0 mg a.i./L of abamectin. The micronucleus test and the tests applied to other nuclear abnormalities in the peripheral blood of birds were conducted 40 days after exposure. Our study revealed significant physical abnormalities in nuclear shapes (erythrocytes with asymmetric constriction nuclei, notched nuclei, indented and moved nucleus) of those birds exposed to higher abamectin levels. When all nuclear abnormalities were tallied, a significant dose-dependent trend was noted. Therefore, our study presents initial imprints on determination of abamectin-mediated cellular toxicity in avifauna which can be instrumental in checking polluted ecosystems.
虽然先前的研究已经证实了阿维菌素（ABA）在不同实验模型（从无脊椎动物到脊椎动物）中的毒理学潜力，但几乎没有关于暴露于这种农药会对鸟类造成什么影响的研究。因此，我们的研究旨在调查低浓度阿维菌素暴露对雌性日本鹌鹑（Coturnix japonica）红细胞的细胞毒性作用。为此，我们提出了三个实验组：“对照组”，由暴露于不含阿维菌素饮用水的鹌鹑组成；“ABA 1%半数致死剂量（LD）组”，由暴露于含有 15.5mg a.i./L 阿维菌素的水中的鸟类组成（通过商业制剂 Kraft® 36EC），以及“ABA 10% LD 组”，由暴露于含有 155.0mg a.i./L 阿维菌素的水中的鸟类组成。暴露 40 天后，对鸟类外周血中的微核试验和其他核异常进行了检测。我们的研究表明，暴露于较高阿维菌素水平的鸟类的核形状出现了明显的物理异常（具有不对称收缩核、缺口核、凹陷和移位核的红细胞）。当所有核异常都被计算在内时，发现存在显著的剂量依赖性趋势。因此，我们的研究初步确定了阿维菌素介导的鸟类细胞毒性，这对于检查受污染的生态系统具有重要意义。
