Wnt 信号通路在角化棘皮瘤中的作用。
Role of the Wnt signaling pathway in keratoacanthoma.
机构信息
Department of Pathology, Rikshospitalet, Oslo University Hospital, Oslo, Norway.
Institute of Clinical Medicine, Department of Pathology, Akershus University Hospital, Lørenskog, Norway.
出版信息
Cancer Rep (Hoboken). 2020 Apr;3(2):e1219. doi: 10.1002/cnr2.1219. Epub 2019 Nov 11.
BACKGROUND
Keratoacanthoma (KA) has a unique life cycle of rapid growth and spontaneous regression that shows similarities to the hair follicle cycle, which involves an active Wnt signaling during physiological regeneration. We analyzed the expression of the Wnt signaling proteins β-catenin, Lef1, Sox9, and Cyclin D1 in young and old human KAs to investigate a possible role for Wnt signaling in KAs.
AIM
To investigate the role of the Wnt/β-catenin signaling pathway in human KAs.
METHODS AND RESULTS
Formalin-fixed, paraffin-embedded tissue samples of 67 KAs were analyzed for protein expression using immunohistochemistry. The majority of KAs were positive for Sox9 and Cyclin D1 but not for nuclear-localized β-catenin or Lef-1. No significant differences in protein expressions were seen between young and old KAs. However, we found a significant association between Ki67 and Cyclin D1 proteins (P= .008).
CONCLUSIONS
The Wnt signaling pathway does not appear to play a significant role in the biogenesis of human KA. Sox9 overexpression may be indicative of inhibition of Wnt signaling. Sox-9 and Cyclin D1 are proliferation markers that are most likely transactivated by alternate signaling pathways.
背景
角化棘皮瘤（KA）具有快速生长和自发消退的独特生命周期，这与涉及生理再生过程中活跃的 Wnt 信号的毛囊周期相似。我们分析了年轻和老年人类 KA 中 Wnt 信号蛋白β-连环蛋白、Lef1、Sox9 和 Cyclin D1 的表达，以研究 Wnt 信号在 KA 中的可能作用。
目的
研究 Wnt/β-连环蛋白信号通路在人类 KA 中的作用。
方法和结果
使用免疫组织化学法分析了 67 例 KA 的福尔马林固定、石蜡包埋组织样本的蛋白表达。大多数 KA 对 Sox9 和 Cyclin D1 呈阳性，但对核定位的β-连环蛋白或 Lef-1 呈阴性。年轻和老年 KA 之间的蛋白表达无显著差异。然而，我们发现 Ki67 和 Cyclin D1 蛋白之间存在显著相关性（P=.008）。
结论
Wnt 信号通路似乎在人类 KA 的发生中没有起重要作用。Sox9 的过度表达可能表明 Wnt 信号受到抑制。Sox-9 和 Cyclin D1 是增殖标志物，很可能通过替代信号通路被反式激活。
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