Ben-Ishay Z, Sharon S
Isr J Med Sci. 1977 Apr;13(4):385-93.
An ultrastructural study of diffusion chamber (DC) hematopoietic colonies derived from rat bone marrow is reported. The emphasis is placed on the structural features of macrophages and fibroblasts in cultures. The observations indicate that the two cell types are normal components of DC cultures. In older cultures both macrophages and fibroblasts contain large lipid droplets, sometimes causing difficulty in the precise characterization of the cell type. The possible origin of fibroblasts from adventitial cells if sinuses or of larger blood vessels of the bone marrow implanted in diffusion chambers is discussed. It is suggested that fibroblasts exert an enhancing influence on the growth of blood cells in DC cultures, comparable to feeder layers in in vitro cultures.
本文报道了对源自大鼠骨髓的扩散室（DC）造血集落的超微结构研究。重点在于培养物中巨噬细胞和成纤维细胞的结构特征。观察结果表明，这两种细胞类型是DC培养物的正常组成部分。在较老的培养物中，巨噬细胞和成纤维细胞都含有大的脂滴，有时难以精确鉴定细胞类型。文中讨论了成纤维细胞可能来源于植入扩散室的骨髓窦的外膜细胞或较大血管的外膜细胞。有人提出，成纤维细胞对DC培养物中血细胞的生长有促进作用，类似于体外培养中的饲养层。
