Nandabalan K, Padayatty J D
Centre for Genetic Engineering, Indian Institute of Science, Bangalore.
Biochem Biophys Res Commun. 1988 Nov 30;157(1):68-74. doi: 10.1016/s0006-291x(88)80012-3.
A clone bearing a 9.8 kb insert DNA containing the rDNA unit was identified by screening an EcoR1 library of rice DNA in lambda Charon 4 phage with [32P]-rRNAs. The S1 nuclease mapping of the rDNA-precursor rRNA hybrids showed the presence of two transcription termini on the rDNA. They were mapped at positions 616 and 620 nucleotides downstream to the end of the 25S rRNA gene. The 18 nucleotide sequence, where the transcription terminates on the rDNA in rice and mice are homologous albeit in the reverse orientation.
通过用[32P] -rRNAs筛选λ噬菌体Charon 4中的水稻DNA的EcoR1文库,鉴定出一个携带包含rDNA单元的9.8 kb插入DNA的克隆。rDNA -前体rRNA杂种的S1核酸酶图谱显示rDNA上存在两个转录末端。它们被定位在25S rRNA基因末端下游616和620个核苷酸处。水稻和小鼠rDNA上转录终止的18个核苷酸序列是同源的,尽管方向相反。