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水稻中核糖体DNA转录的假定终止位点。

Putative termination sites for rDNA transcription in rice.

作者信息

Nandabalan K, Padayatty J D

机构信息

Centre for Genetic Engineering, Indian Institute of Science, Bangalore.

出版信息

Biochem Biophys Res Commun. 1988 Nov 30;157(1):68-74. doi: 10.1016/s0006-291x(88)80012-3.

Abstract

A clone bearing a 9.8 kb insert DNA containing the rDNA unit was identified by screening an EcoR1 library of rice DNA in lambda Charon 4 phage with [32P]-rRNAs. The S1 nuclease mapping of the rDNA-precursor rRNA hybrids showed the presence of two transcription termini on the rDNA. They were mapped at positions 616 and 620 nucleotides downstream to the end of the 25S rRNA gene. The 18 nucleotide sequence, where the transcription terminates on the rDNA in rice and mice are homologous albeit in the reverse orientation.

摘要

通过用[32P] -rRNAs筛选λ噬菌体Charon 4中的水稻DNA的EcoR1文库,鉴定出一个携带包含rDNA单元的9.8 kb插入DNA的克隆。rDNA -前体rRNA杂种的S1核酸酶图谱显示rDNA上存在两个转录末端。它们被定位在25S rRNA基因末端下游616和620个核苷酸处。水稻和小鼠rDNA上转录终止的18个核苷酸序列是同源的,尽管方向相反。

相似文献

1
Putative termination sites for rDNA transcription in rice.水稻中核糖体DNA转录的假定终止位点。
Biochem Biophys Res Commun. 1988 Nov 30;157(1):68-74. doi: 10.1016/s0006-291x(88)80012-3.
2
A minor 9.8 kb rDNA unit of rice variety IR-20.
Indian J Biochem Biophys. 1989 Oct;26(5):289-92.
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Initiation of transcription of rDNA in rice.水稻核糖体DNA转录的起始
Biochem Biophys Res Commun. 1989 May 15;160(3):1117-23. doi: 10.1016/s0006-291x(89)80119-6.

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