Iskierka M, Zawadzki M, Szpot P, Jurek T
Department of Forensic Medicine, Wroclaw Medical University, J. Mikulicza-Radeckiego 4 50-367 Wrocław 71 784 14 58, Poland.
J Anal Toxicol. 2021 Apr 12;45(4):348-355. doi: 10.1093/jat/bkaa083.
In case of basic biological materials (blood, urine, vitreous humor) being unavailable, bone marrow can be used for toxicological tests. The aim of the study was to assess the concentration of various xenobiotics in the bone marrow, as well as in the blood and the vitreous humor. The analysis was performed in the biological material originating from the autopsy (n = 120), using the LC-MS method and with liquid/liquid extraction at pH = 9. As many as 46 different xenobiotics were detected in the biological material, strong correlations between the concentration of a given xenobiotic in blood and bone marrow, as well as in the vitreous humor and bone marrow, were noted for most of them, with the exception of diazepam and 7-aminoclonazepam. The obtained results indicate the possibility of using bone marrow to determine the concentration of numerous xenobiotics in the situation of basic biological materials being unavailable, as well as using the results obtained in the future to better understand the pharmacokinetic processes and the effect of postmortem redistribution on medication and drug concentrations in the body of a deceased person.
如果无法获取基础生物材料（血液、尿液、玻璃体液），骨髓可用于毒理学检测。本研究的目的是评估骨髓以及血液和玻璃体液中各种外源性物质的浓度。分析是在源自尸检的生物材料（n = 120）中进行的，采用液相色谱 - 质谱法，并在pH = 9的条件下进行液 - 液萃取。在生物材料中检测到多达46种不同的外源性物质，除地西泮和7 - 氨基氯硝西泮外，大多数外源性物质在血液与骨髓、玻璃体液与骨髓中的浓度之间均存在强相关性。所得结果表明，在无法获取基础生物材料的情况下，利用骨髓来测定多种外源性物质浓度具有可能性，并且利用未来获得的结果能够更好地理解药代动力学过程以及死后再分布对死者体内药物和毒品浓度的影响。
