Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, Heilongjiang 150040, People's Republic of China.
Engineering Research Center of Forest Bio-Preparation, Ministry of Education, Northeast Forestry University, Harbin, Heilongjiang 150040, People's Republic of China.
J Agric Food Chem. 2020 Aug 5;68(31):8350-8361. doi: 10.1021/acs.jafc.0c02600. Epub 2020 Jul 28.
Phenolic compounds in pigeon pea possess various biological properties beneficial to human health. In this study, pigeon pea hairy root cultures (PPHRCs) were developed as an effective platform for the production of phenolic compounds. A high-productive hairy root line was screened and characterized, and its culture conditions were optimized in terms of biomass productivity and phenolic yield. The comparative profiling of 10 phenolic compounds in PPHRCs and pigeon pea natural resources (seeds, leaves, and roots) was achieved by ultra-high-performance liquid chromatography-tandem mass spectrometry analysis. The total phenolic yield in PPHRCs (3278.44 μg/g) was much higher than those in seeds (68.86 μg/g) and roots (846.03 μg/g), and comparable to leaves (3379.49 μg/g). Notably, PPHRCs exhibited superiority in the yield of the most important health-promoting compound cajaninstilbene acid (2996.23 μg/g) against natural resources (4.42-2293.31 μg/g). Overall, PPHRCs could serve as promising potential alternative sources for the production of phenolic compounds with nutraceutical/medicinal values.
兵豆中的酚类化合物具有多种有益于人类健康的生物特性。在这项研究中，兵豆毛状根培养物（PPHRCs）被开发为生产酚类化合物的有效平台。筛选并表征了高产毛状根系，并优化了其培养条件，以提高生物量生产力和酚类产量。通过超高效液相色谱-串联质谱分析，对 PPHRCs 和兵豆天然资源（种子、叶子和根）中的 10 种酚类化合物进行了比较分析。PPHRCs 中的总酚产量（3278.44 μg/g）远高于种子（68.86 μg/g）和根（846.03 μg/g），与叶子（3379.49 μg/g）相当。值得注意的是，PPHRCs 在最具健康促进作用的化合物 cajaninstilbene acid 的产量方面具有优势（2996.23 μg/g），而天然资源中的含量为 4.42-2293.31 μg/g。总的来说，PPHRCs 可以作为具有营养/药用价值的酚类化合物生产的有前途的潜在替代来源。
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