Magrini Sara, Azzella Mattia M, Bolpagni Rossano, Zucconi Laura
Tuscia Germplasm Bank, Tuscia University, largo dell'Università-blocco C, 01100 Viterbo, Italy.
National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA)-Frascati Research Center, Via Enrico Fermi 45, 00044 Frascati, Italy.
Plants (Basel). 2020 Jul 14;9(7):887. doi: 10.3390/plants9070887.
is an aquatic quillwort endemic to Italy. It is one of the rarest quillworts in Europe, and is critically endangered due to restricted range and to the continuous decline of both population and habitat quality. This study aims to develop an optimized protocol to reproduce and grow sporelings. Mature and immature megaspores were mixed with mature microspores to evaluate the influence of the developmental stage on germination and sporeling development. Two substrates, distilled water and water-agar medium, were tested for germination and sporeling emergence, and three substrates, sand, lake sediment and water-agar, were tested for transplants. A high percentage of megaspore germination (a total of 79.1%) was obtained in both substrates, higher for mature than immature spores. A total of 351 sporelings were produced in distilled water and water-agar cultures, with similar percentages (64.5% and 69.6%, respectively). The development stage of the megaspores affected both germination and sporeling development. Sporeling emergence showed significantly higher percentages in mature megaspores than immature ones (69.6% vs. 11.6%, respectively), with 85% of germinated spores developing sporelings. Only transplants over water-agar medium were successful. This protocol could be useful for the propagation of sporelings as the key step towards the planning of in situ actions to save this Mediterranean quillwort from extinction.
是一种意大利特有的水生水韭。它是欧洲最稀有的水韭之一，由于分布范围有限以及种群数量和栖息地质量持续下降而处于极度濒危状态。本研究旨在制定一种优化方案来繁殖和培育幼苗。将成熟和未成熟的大孢子与成熟的小孢子混合，以评估发育阶段对萌发和幼苗发育的影响。测试了两种用于萌发和幼苗出土的基质，即蒸馏水和水琼脂培养基，并测试了三种用于移植的基质，即沙子、湖底沉积物和水琼脂。在两种基质中都获得了较高百分比的大孢子萌发率（总计79.1%），成熟孢子的萌发率高于未成熟孢子。在蒸馏水和水琼脂培养中总共产生了351株幼苗，百分比相似（分别为64.5%和69.6%）。大孢子的发育阶段影响萌发和幼苗发育。成熟大孢子中的幼苗出土率明显高于未成熟大孢子（分别为69.6%和11.6%），85%的萌发孢子发育成幼苗。只有在水琼脂培养基上的移植成功。该方案对于幼苗繁殖可能有用，这是规划原地行动以拯救这种地中海水韭免于灭绝的关键步骤。
