Department of Thoracic-cardiovascular Surgery, Shanghai Jiaotong University Affiliated Sixth Hospital , Shanghai, China.
Organogenesis. 2020 Jul 2;16(3):73-82. doi: 10.1080/15476278.2020.1790273. Epub 2020 Jul 16.
Tracheal reconstruction after extensive resection remains a challenge in thoracic surgery. Aortic allograft has been proposed to be a potential tracheal substitute. However, clinically, its application is limited for the shortage of autologous aortic segment. Whether xenogeneic aortic biosheets can be used as tracheal substitutes remains unknown. In the present study, we investigated the possibility in dog model. The results show that all dogs were survived without airway symptoms at 6 months after tracheal reconstruction with gently decellularized bovine carotid arteries. In the interior of engrafted areas, grafted patch integrated tightly with the residual native tracheal tissues and tracheal defects in the lumen were repaired smoothly without obvious inflammation, granulation, anastomotic leakage, or stenosis. In addition, histological and scanning electron microscopy examination showed that grafted patches were covered with ciliated columnar epithelium similar to epithelium in native trachea, which indicated successfully re-epithelialization of decellularized bovine carotid arteries in dogs. These findings provide preclinical investigation of xenogeneic aortic biosheets in serving as tracheal substitute in a dog model, which proposes that decellularized biosheets of bovine carotid may be a potential material for bioartificial tracheal graft.
广泛切除后的气管重建仍然是胸外科的一个挑战。同种异体主动脉已被提议作为潜在的气管替代物。然而，由于自体主动脉节段的短缺，其临床应用受到限制。异种主动脉生物瓣是否可以用作气管替代品仍不清楚。在本研究中，我们在犬模型中进行了研究。结果显示，在经过轻度去细胞化牛颈动脉气管重建 6 个月后，所有犬均存活，无气道症状。在植入区域的内部，移植物与残余的天然气管组织紧密结合，管腔中的气管缺损得到了顺利修复，没有明显的炎症、肉芽组织、吻合口漏或狭窄。此外，组织学和扫描电子显微镜检查显示，移植物被类似于天然气管的纤毛柱状上皮覆盖，这表明去细胞化牛颈动脉在犬中成功地再上皮化。这些发现为异种主动脉生物瓣在犬模型中作为气管替代物提供了临床前研究，表明去细胞化牛颈动脉生物瓣可能是生物人工气管移植物的一种潜在材料。
