UIC/NIH Center for Botanical Dietary Supplements Research, Program for Collaborative Research in the Pharmaceutical Sciences (PCRPS), Department of Pharmaceutical Sciences, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, United States.
UIC/NIH Center for Botanical Dietary Supplements Research, Program for Collaborative Research in the Pharmaceutical Sciences (PCRPS), Department of Pharmaceutical Sciences, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612, United States.
Fitoterapia. 2020 Oct;146:104686. doi: 10.1016/j.fitote.2020.104686. Epub 2020 Jul 15.
The H NMR spectra of crude extracts from a total of 33 Actaea samples were acquired and analyzed for their species- and plant part-specific metabolomic characteristics by identifying fingerprint resonances via visual observation as well as a chemometric approach using principal component analysis (PCA). The main study subjects were the roots/rhizomes and aerial parts of three American species, Actaea racemosa (AR), Actaea podocarpa (AP) and Actaea cordifolia (AC). AP exhibited an already visually distinct chemical profile from those of the other two species. The species-characteristic resonances were identified as analytical chemotaxonomic markers. AR and AC exhibited visually similar H NMR spectral profiles that required statistical analysis for differentiation. Several characteristic peaks and peak patterns were identified for each group of samples. Together with the three American Actaea species, the characteristics of the H NMR spectra of Asian species are also discussed. A statistical analysis method using PCA was employed to provide the metabolomic profile for visually minor but analytically significant chemotaxonomic differences. PCA scores allowed differentiation between the three American Actaea species, as well as the ability to differentiate between the various plant parts (aboveground vs. roots/rhizomes).
对总共 33 个白头翁属样本的粗提物进行了 1 H NMR 谱分析，并通过对指纹共振的直观观察和主成分分析（PCA）等化学计量学方法，对其具有物种和植物部位特异性的代谢组学特征进行了分析。主要研究对象为三种美洲种白头翁属植物（AR）、Actaea podocarpa（AP）和 Actaea cordifolia（AC）的根/根茎和地上部分。AP 的化学特征与其他两种植物明显不同。鉴定出的特征共振可作为分析化学分类标记。AR 和 AC 的 1 H NMR 光谱图谱在视觉上相似，需要进行统计分析以进行区分。对每组样本都鉴定出了几个特征峰和峰型。结合三种美洲白头翁属植物，还讨论了亚洲种的 1 H NMR 图谱特征。采用 PCA 等统计分析方法，为具有视觉差异但在分析上具有重要意义的化学分类差异提供代谢组学图谱。PCA 得分不仅可以区分三种美洲白头翁属植物，还可以区分不同的植物部位（地上部分与根/根茎）。
