Bioprocessing and Biodetection Laboratory, Department of Food Science and Engineering, University of Tehran, Karaj, Iran.
Health Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran; Department of Nutrition and Food Hygiene, Faculty of Health, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Int J Biol Macromol. 2020 Dec 1;164:1025-1036. doi: 10.1016/j.ijbiomac.2020.07.107. Epub 2020 Jul 14.
The current work investigated a simultaneous recovery of pectin and polyphenols from black mulberry pomace (BMP) using an eco-friendly extraction process. The microwave-assisted extraction variables were successfully co-optimized using Box-Behnken design considering pectin and polyphenols yields as responses. The optimized condition yielded about 10.95% pectin and 12.11% phenolics. The physicochemical analysis indicated a highly-esterified pectin (DE of 62.21%) with an average molecular weight of 620.489 kDa and galacturonic acid (GalA) content of 70.15%. FTIR and NMR spectroscopies confirmed the predominant presence of highly esterified GalA-rich structure for BMP pectin. XRD analysis suggested an amorphous structure for the main part of BMP pectin. Also, DSC results showed higher thermal stability for BMP pectin in comparison to commercial pectin. Moreover, the antiradical activity of BMP phenolic extract was very close to that of butylated hydroxyanisole (BHA) and ascorbic acid. The results showed that the suggested procedure can be a promising solution for the management of BMP waste generated in juice, syrup, or liquor processing plants. Also, the quality of the obtained products (BMP pectin and BMP phenolic extract) indicated that the products have the potential to be used as natural ingredients in various food and pharmaceutical products.
本研究采用环保提取工艺，从黑桑椹渣（BMP）中同时回收果胶和多酚。通过 Box-Behnken 设计，成功地对微波辅助提取变量进行了协同优化，以果胶和多酚的产率作为响应。优化条件下，果胶得率约为 10.95%，多酚得率约为 12.11%。理化分析表明，该果胶具有高度酯化特性（酯化度为 62.21%），平均分子量为 620.489 kDa，半乳糖醛酸（GalA）含量为 70.15%。傅里叶变换红外光谱（FTIR）和核磁共振（NMR）光谱证实了 BMP 果胶主要为高度酯化的 GalA 丰富结构。X 射线衍射（XRD）分析表明 BMP 果胶的主要部分呈无定形结构。此外，差示扫描量热法（DSC）结果表明，与商业果胶相比，BMP 果胶具有更高的热稳定性。此外，BMP 类多酚提取物的自由基清除活性非常接近丁基羟基茴香醚（BHA）和抗坏血酸。结果表明，所提出的方法可以作为果汁、糖浆或酒加工厂产生的 BMP 废物管理的一种有前途的解决方案。此外，所获得的产品（BMP 果胶和 BMP 类多酚提取物）的质量表明，这些产品具有作为天然成分在各种食品和制药产品中应用的潜力。
