The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, 310053, PR China.
Department of Urology and Andrology, Affiliated Hospital of Nantong University, Nantong, 226001, PR China.
Biomed Pharmacother. 2020 Oct;130:110405. doi: 10.1016/j.biopha.2020.110405. Epub 2020 Jul 14.
Neurogenic erectile dysfunction (NED) is an inevitable postoperative disease of cavernous nerve injury which will lead to various pathophysiological changes in the corpus cavernosum and dorsal penile nerve caused by radical prostatectomy (RP). Although serval years of clinical application of HJIG I granules (HJIG), an innovative formulation, has demonstrated its reliable clinical efficacy against NED, the mechanism of HJIG remains unclear. This study aimed to assess the neuroprotective effect of HJIG, to repair damaged nerves in a rat model of bilateral cavernous nerve injury (BCNI) in vivo and their effects on neurites of major pelvic ganglia (MPG) regeneration and Schwann cells (SCs) proliferation in vitro. Rats were divided into five groups randomly: normal control (NC), BCNI-induced ED model (M), M + low-dose HJIG (HL), M + medium-dose HJIG (HM), and M + high-dose HJIG (HH). All groups were treated with normal saline or the relevant drug for 28 consecutive days after a standard NED animal model. Our data revealed that administration of HJIG improved NED that was detected by intracavernous pressure (ICP) in a dose-dependent manner. The haematoxylin-eosin (HE) and Immunofluorescence (IF) staining demonstrated that HJIG ameliorate the shape of penis and induced the protein synthesis of GAP43, NF200, S100, and nNOS. NF200 and S100 level were also detected by western blotting. Moreover, HJIG (0.78 mg/mL) markedly increased SCs viability and promoted neurites regeneration of MPG. These findings provide new insights into the NED therapy by HJIG.
神经源性勃起功能障碍（NED）是海绵体神经损伤根治性前列腺切除术后不可避免的术后疾病，会导致海绵体和阴茎背神经发生各种病理生理变化。尽管 HJIG I 颗粒（HJIG）这种创新制剂的临床应用已有数年，已证明其对 NED 的可靠临床疗效，但 HJIG 的作用机制尚不清楚。本研究旨在评估 HJIG 的神经保护作用，研究其在体内双侧海绵体神经损伤（BCNI）大鼠模型中修复受损神经的作用，以及在体外对主要骨盆神经节（MPG）再生和雪旺细胞（SCs）增殖的影响。大鼠随机分为五组：正常对照组（NC）、BCNI 诱导的 ED 模型组（M）、M+低剂量 HJIG 组（HL）、M+中剂量 HJIG 组（HM）和 M+高剂量 HJIG 组（HH）。所有组均在建立标准 NED 动物模型后连续 28 天用生理盐水或相关药物治疗。我们的数据表明，HJIG 给药可改善依赖于剂量的 ICP 检测到的 NED。苏木精-伊红（HE）和免疫荧光（IF）染色表明，HJIG 改善了阴茎的形状，并诱导了 GAP43、NF200、S100 和 nNOS 的蛋白合成。还通过 Western blot 检测了 NF200 和 S100 水平。此外，HJIG（0.78mg/mL）显著提高了SCs 的活力，并促进了 MPG 神经突的再生。这些发现为 HJIG 治疗 NED 提供了新的思路。
