在附着于可操控基质的离体垂体细胞中研究促黄体生成素分泌的短期动力学。
Short term kinetics of luteinizing hormone secretion studied in dissociated pituitary cells attached to manipulable substrates.
作者信息
Hopkins C R
出版信息
J Cell Biol. 1977 Jun;73(3):685-95. doi: 10.1083/jcb.73.3.685.
Abstract
With the use of poly-L-lysine, a method has been developed which induces acutely dissociated rat anterior pituitary cells to attach to glass and polyacrylamide surfaces. In these attached cells the recovery of the secretory response, which is impaired in acutely dissociated cells, has been followed, and it has been established that, in terms of their ability to secrete luteinizing hormone (LH) in response to the specific secretogogue luteinizing-hormone-releasing hormone (LHRH), the cells become maximally responsive after 48 h. The attached cells also allow the short-term kinetics of LH secretion to be followed with great facility; and, when cells allowed to recover for 48 h are used, it is shown that in response to LHRH the pattern of LH release is biphasic.
摘要
通过使用聚-L-赖氨酸，已开发出一种方法，可诱导急性解离的大鼠垂体前叶细胞附着于玻璃和聚丙烯酰胺表面。在这些附着的细胞中，研究了急性解离细胞中受损的分泌反应的恢复情况，并且已经确定，就其响应特异性促分泌素促黄体生成激素释放激素（LHRH）分泌促黄体生成激素（LH）的能力而言，细胞在48小时后反应达到最大。附着的细胞还便于轻松跟踪LH分泌的短期动力学；并且，当使用恢复48小时的细胞时，结果表明，响应LHRH时，LH释放模式是双相的。
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本文引用的文献
1
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Endocrinology. 1968 Nov;83(5):1004-12. doi: 10.1210/endo-83-5-1004.
2
The derivatization of cross-linked polyacrylamide beads. Controlled introduction of functional groups for the preparation of special-purpose, biochemical adsorbents.交联聚丙烯酰胺微球的衍生化。为制备特殊用途的生化吸附剂而对官能团进行的可控引入。
Biochemistry. 1969 Oct;8(10):4074-82. doi: 10.1021/bi00838a026.
3
Evidence for a periodic release of LH in castrated male and female rats.
Endocrinology. 1972 Jan;90(1):158-62. doi: 10.1210/endo-90-1-158.
4
Some effects of energy-transfer inhibitors and of Ca++-free or K+-enhanced media on the release of luteinizing hormone (LH) from the rat pituitary gland in vitro.
Endocrinology. 1968 Feb;82(2):225-31. doi: 10.1210/endo-82-2-225.
5
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6
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7
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