Martínez Tomás Francisco, Alcalde María Jesús, Sáez María Isabel, Suárez María Dolores
Departamento de Biología y Geología, Universidad de Almería, 04120 Almería, Spain.
Departamento de Ciencias Agroforestales, Universidad de Sevilla, 41013 Sevilla, Spain.
Foods. 2020 Jul 15;9(7):934. doi: 10.3390/foods9070934.
The combined effect of farm management practices, transport time, and ageing time on the electrophoretic changes of sarcoplasmic (SPP) and myofibrillar (MFP) protein fractions of goat kids was studied. A total of 64 suckling goat kids were withdrawn from two farms with "high" (GW) and "low" (DW) welfare-friendly management practices, and they were transported for 2 or 6 h immediately before slaughtering. samples were obtained at 3, 8, and 21 days post-mortem, and muscle proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis SDS-PAGE. Both protein extracts displayed significant changes attributable to meat maturation. Managing conditions of kids in DW farms increased the post-mortem susceptibility of muscle proteins. Some MFP of muscle, such as troponin T, as well as 26-30 and 35-37 kDa fractions were influenced significantly by deficient on-farm management, and therefore, these protein fragments might be considered as indicators of low-welfare on-farm management in goat kids.
研究了养殖管理方式、运输时间和老化时间对奶山羊肌浆蛋白(SPP)和肌原纤维蛋白(MFP)组分电泳变化的综合影响。从两个采用“高”(GW)和“低”(DW)福利友好型管理方式的农场中选取了64只哺乳奶山羊,在屠宰前立即运输2或6小时。在宰后3、8和21天采集样本,并用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)分离肌肉蛋白。两种蛋白质提取物均显示出因肉成熟而产生的显著变化。DW农场中奶山羊的管理条件增加了肌肉蛋白宰后的敏感性。肌肉中的一些肌原纤维蛋白,如肌钙蛋白T,以及26 - 30 kDa和35 - 37 kDa组分受农场管理不善的影响显著,因此,这些蛋白片段可被视为奶山羊低福利农场管理的指标。