Key Laboratory of Structure and Functional Regulation of Hybrid Materials (Ministry of Education), Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry & Chemical Engineering, Anhui University, Hefei, Anhui, 230601, People's Republic of China.
Mikrochim Acta. 2020 Jul 18;187(8):455. doi: 10.1007/s00604-020-04425-1.
An ultrasensitive electrochemiluminescence biosensor was established based on the Zn-MOF/GO nanocomposite. Ag(I)-embedded DNA complexes were used as a signal amplification reagent. In this work, 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin (TCPP) and Zn were integrated into a porphyrin paddlewheel framework (Zn-MOF) by a hydrothermal method. The synthesized Zn-MOF material has electrochemiluminescence property, and the luminescence intensity is improved after being composited with graphene oxide (GO). Based on the composite material, we constructed an ultrasensitive ECL biosensor for the p53 antibody detection. The composite material acted as an admirable substrate and then loaded plenty of p53 antigens to recognize the target (p53 antibody) accurately. Because of the bridging effect of streptavidin and biotin-conjugated goat anti-rabbit IgG (bio-ab2), the rich-C DNA with positive correlation with the target was modified on the electrode and then captured the co-reactant accelerator Ag to amplify the signal. Therefore, the ECL biosensor response increases with increasing p53 antibody concentration. In the range 0.1 fg/mL-0.01 ng/mL, the response signal of the biosensor has a good linear relationship with the p53 antibody concentration. The detection limit is 0.03 fg/mL (S/N = 3). Impressively, the biosensor not only featured high sensitivity, good stability, and excellent specificity for the detection of p53 antibody, but also provides a new way for early detection of cancer. Graphical abstract Schematic representation of the electrochemiluminescence sensor based on a Zn-MOF/GO nanocomposite, which can be applied to the determination of p53 antibody.
基于 Zn-MOF/GO 纳米复合材料建立了一种超灵敏的电化学发光生物传感器。Ag(I)-嵌入的 DNA 复合物被用作信号放大试剂。在这项工作中，通过水热法将 5,10,15,20-四(4-羧基苯基)卟啉 (TCPP) 和 Zn 整合到卟啉桨轮框架 (Zn-MOF) 中。合成的 Zn-MOF 材料具有电化学发光性能，与氧化石墨烯 (GO) 复合后发光强度提高。基于该复合材料，我们构建了一种用于 p53 抗体检测的超灵敏 ECL 生物传感器。该复合材料作为一种理想的基底，然后负载大量的 p53 抗原，以准确识别目标 (p53 抗体)。由于链霉亲和素和生物素化兔抗山羊 IgG (bio-ab2) 的桥接效应，与靶标呈正相关的富含 C 的 DNA 被修饰在电极上，然后捕获共反应物加速剂 Ag 以放大信号。因此，ECL 生物传感器的响应随 p53 抗体浓度的增加而增加。在 0.1 fg/mL-0.01 ng/mL 范围内，生物传感器的响应信号与 p53 抗体浓度呈良好的线性关系。检测限为 0.03 fg/mL (S/N = 3)。令人印象深刻的是，该生物传感器不仅对 p53 抗体的检测具有高灵敏度、良好的稳定性和优异的特异性，而且为癌症的早期检测提供了一种新方法。
